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  Triarylmethane fluorophores resistant to oxidative photobluing.

Butkevich, A., Bossi, M. L., Lukinavicius, G., & Hell, S. W. (2019). Triarylmethane fluorophores resistant to oxidative photobluing. Journal of the American Chemical Society, 141(2), 981-989. doi:10.1021/jacs.8b11036.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-B3BD-F Version Permalink: http://hdl.handle.net/21.11116/0000-0003-4719-2
Genre: Journal Article

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 Creators:
Butkevich, A.1, Author              
Bossi, M. L.1, Author              
Lukinavicius, G.2, Author              
Hell, S. W.1, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              
2Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society, ou_2616691              

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 Abstract: Spectral stability of small-molecule fluorescent probes is required for correct interpretation and reproducibility of multicolor fluorescence imaging data, in particular under high (de)excitation light intensities of super-resolution imaging or in single-molecule applications. We propose a synthetic approach to a series of spectrally stable rhodamine fluorophores based on sequential Ru- and Cu-catalyzed transformations, evaluate their stability against photobleaching and photoconversion in context of other fluorophores using chemometric analysis, and demonstrate chemical reactivity of fluorophore photoproducts. The substitution patterns providing the photoconversion-resistant triarylmethane fluorophores have been identified, and the applicability of non-bluing labels in live-cell STED nanoscopy is demonstrated.

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Language(s): eng - English
 Dates: 2018-12-182019-01-16
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1021/jacs.8b11036
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Title: Journal of the American Chemical Society
Source Genre: Journal
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Pages: - Volume / Issue: 141 (2) Sequence Number: - Start / End Page: 981 - 989 Identifier: -