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  The occurrence and formation of monoterpenes in herbivoredamaged poplar roots

Lackus, N., Lackner, S., Gershenzon, J., Unsicker, S., & Köllner, T. G. (2018). The occurrence and formation of monoterpenes in herbivoredamaged poplar roots. Scientific Reports, 8: 17936. doi:10.1038/s41598-018-36302-6.

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http://dx.doi.org/10.1038/s41598-018-36302-6 (Publisher version)
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 Creators:
Lackus, Nathalie1, 2, Author              
Lackner, Sandra1, 2, Author              
Gershenzon, Jonathan1, Author              
Unsicker, Sybille1, Author              
Köllner, Tobias G.1, Author              
Affiliations:
1Department of Biochemistry, Prof. J. Gershenzon, MPI for Chemical Ecology, Max Planck Society, ou_421893              
2IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society, Jena, DE, ou_421900              

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 Abstract: Volatiles are often released upon herbivory as plant defense compounds. While the formation of volatiles above-ground has been intensively studied, little is known about herbivore-induced root volatiles. Here, we show that cockchafer larvae-damaged roots of Populus trichocarpa and P. nigra release a mixture of monoterpenes, including (−)-α-pinene, (−)-camphene, (−)-β-pinene, p-cymene, and 1,8-cineole. Three terpene synthases, PtTPS16 and PtTPS21 from P. trichocarpa and PnTPS4 from P. nigra, could be identified and characterized in vitro. PnTPS4 was found to produce 1,8-cineole as sole product. PtTPS16 and PtTPS21, although highly similar to each other, showed different product specificities and produced γ-terpinene and a mixture of (−)-camphene, (−)-α-pinene, (−)-β-pinene, and (−)-limonene, respectively. Four active site residues were found to determine the different product specificities of the two enzymes. The expression profiles of PtTPS16, PtTPS21, and PnTPS4 in undamaged and herbivore-damaged poplar roots generally matched the emission pattern of monoterpenes, indicating that monoterpene emission in roots is mainly determined at the gene transcript level. Bioassays with Phytophtora cactorum (Oomycetes) revealed inhibitory effects of vaporphase 1,8-cineole and (−)-β-pinene on the growth of this important plant pathogen. Thus herbivoreinduced volatile monoterpenes may have a role in defense against pathogens that cause secondary infections after root wounding.

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 Dates: 2018-11-142018-12-18
 Publication Status: Published online
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 Identifiers: Other: GER537
DOI: 10.1038/s41598-018-36302-6
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Title: Scientific Reports
  Abbreviation : Sci. Rep.
Source Genre: Journal
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Publ. Info: London, UK : Nature Publishing Group
Pages: - Volume / Issue: 8 Sequence Number: 17936 Start / End Page: - Identifier: ISSN: 2045-2322
CoNE: https://pure.mpg.de/cone/journals/resource/2045-2322