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  In-depth structural analysis of glycans in the gas phase

Mucha, E., Stuckmann, A., Marianski, M., Struwe, W. B., Meijer, G., & Pagel, K. (2019). In-depth structural analysis of glycans in the gas phase. Chemical Science, 10(5), 1272-1284. doi:10.1039/c8sc05426f.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-FC04-E Version Permalink: http://hdl.handle.net/21.11116/0000-0002-FD18-7
Genre: Journal Article

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 Creators:
Mucha, Eike1, 2, Author              
Stuckmann, Alexandra1, 2, Author              
Marianski, Mateusz1, Author              
Struwe, Weston B.3, Author
Meijer, Gerard1, Author              
Pagel, Kevin1, 2, Author              
Affiliations:
1Molecular Physics, Fritz Haber Institute, Max Planck Society, ou_634545              
2Institute of Chemistry and Biochemistry, Freie Universität Berlin, Takustraße 3, 14195 Berlin, Germany, ou_persistent22              
3Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, OX1 3QU Oxford, UK, ou_persistent22              

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 Abstract: Although there have been substantial improvements in glycan analysis over the past decade, the lack of both high-resolution and high-throughput methods hampers progress in glycomics. This perspective article highlights the current developments of liquid chromatography, mass spectrometry, ion-mobility spectrometry and cryogenic IR spectroscopy for glycan analysis and gives a critical insight to their individual strengths and limitations. Moreover, we discuss a novel concept in which ion mobility-mass spectrometry and cryogenic IR spectroscopy is combined in a single instrument such that datasets consisting of m/z, collision cross sections and IR fingerprints can be obtained. This multidimensional data will then be compared to a comprehensive reference library of intact glycans and their fragments to accurately identify unknown glycans on a high-throughput scale with minimal sample requirements. Due to the complementarity of the obtained information, this novel approach is highly diagnostic and also suitable for the identification of larger glycans; however, the workflow and instrumentation is straightforward enough to be implemented into a user-friendly setup.

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Language(s): eng - English
 Dates: 2018-12-052019-01-042019-01-042019-02-07
 Publication Status: Published in print
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1039/c8sc05426f
 Degree: -

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Title: Chemical Science
  Other : Chem. Sci.
Source Genre: Journal
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Publ. Info: Cambridge, UK : Royal Society of Chemistry
Pages: - Volume / Issue: 10 (5) Sequence Number: - Start / End Page: 1272 - 1284 Identifier: ISSN: 2041-6520
CoNE: https://pure.mpg.de/cone/journals/resource/2041-6520