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  Fluorescent phallotoxin, a tool for the visualization of cellular actin

Wulf, E., Deboben, A., Bautz, F. A., Faulstich, H., & Wieland, T. (1979). Fluorescent phallotoxin, a tool for the visualization of cellular actin. Proceedings of the National Academy of Sciences of the United States of America, 76(9), 4498-4502. doi:10.1073/pnas.76.9.4498.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0003-A3B8-5 Version Permalink: https://hdl.handle.net/21.11116/0000-0003-A3B9-4
Genre: Journal Article

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 Creators:
Wulf , E., Author
Deboben, Axel1, Author           
Bautz, F. A., Author
Faulstich, Heinz2, Author           
Wieland, Theodor1, Author           
Affiliations:
1Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              
2Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society, ou_1497703              

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 Abstract: A fluorescent derivative of phalloidin has been synthesized possessing high affinity to filamentous actin. This compound was used for visualization of actin-containing structures in eukaryotic nonmuscle cells. Due to its low molecular weight (1250), fixation for formaldehyde was sufficient to render the membrane permeable for the labeled peptide. Bundles of microfilaments are the predominant pattern in the flat rat kangaroo PtK1 cells, whereas a net of concentric fibers characterizes the more spherical bovine kidney MDBK cells. Specificity of staining was confirmed by competition experiments with unlabeled phalloidin.

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Language(s): eng - English
 Dates: 1979-06-121979-09-01
 Publication Status: Issued
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1073/pnas.76.9.4498
URI: https://www.ncbi.nlm.nih.gov/pubmed/291981
URI: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC411604/
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Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : Proc. Acad. Sci. USA
  Other : Proc. Acad. Sci. U.S.A.
  Other : Proceedings of the National Academy of Sciences of the USA
  Abbreviation : PNAS
Source Genre: Journal
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Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: 76 (9) Sequence Number: - Start / End Page: 4498 - 4502 Identifier: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230