Fluorescent phallotoxin, a tool for the visualization of cellular actin

Wulf , E.; Deboben, Axel; Bautz, F. A.; Faulstich, Heinz; Wieland, Theodor

doi:10.1073/pnas.76.9.4498

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

タグ情報を表示リリース履歴を表示詳細要約

公開

学術論文

Fluorescent phallotoxin, a tool for the visualization of cellular actin

MPS-Authors

/persons/resource/persons211468

Deboben, Axel
Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons92864

Faulstich, Heinz
Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons197838

Wieland, Theodor
Max Planck Institute for Medical Research, Max Planck Society;

External Resource

https://www.pnas.org/content/pnas/76/9/4498.full.pdf
(全文テキスト（全般）)

https://doi.org/10.1073/pnas.76.9.4498
(全文テキスト（全般）)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

公開されているフルテキストはありません

付随資料 (公開)

There is no public supplementary material available

引用

Wulf, E., Deboben, A., Bautz, F. A., Faulstich, H., & Wieland, T. (1979). Fluorescent phallotoxin, a tool for the visualization of cellular actin. Proceedings of the National Academy of Sciences of the United States of America, 76(9), 4498-4502. doi:10.1073/pnas.76.9.4498.

引用: https://hdl.handle.net/21.11116/0000-0003-A3B8-5

要旨

A fluorescent derivative of phalloidin has been synthesized possessing high affinity to filamentous actin. This compound was used for visualization of actin-containing structures in eukaryotic nonmuscle cells. Due to its low molecular weight (1250), fixation for formaldehyde was sufficient to render the membrane permeable for the labeled peptide. Bundles of microfilaments are the predominant pattern in the flat rat kangaroo PtK1 cells, whereas a net of concentric fibers characterizes the more spherical bovine kidney MDBK cells. Specificity of staining was confirmed by competition experiments with unlabeled phalloidin.