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Journal Article

Fluorescent phallotoxin, a tool for the visualization of cellular actin

MPS-Authors
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Deboben,  Axel
Max Planck Institute for Medical Research, Max Planck Society;

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Faulstich,  Heinz
Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons197838

Wieland,  Theodor
Max Planck Institute for Medical Research, Max Planck Society;

External Resource

https://www.pnas.org/content/pnas/76/9/4498.full.pdf
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https://doi.org/10.1073/pnas.76.9.4498
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Citation

Wulf, E., Deboben, A., Bautz, F. A., Faulstich, H., & Wieland, T. (1979). Fluorescent phallotoxin, a tool for the visualization of cellular actin. Proceedings of the National Academy of Sciences of the United States of America, 76(9), 4498-4502. doi:10.1073/pnas.76.9.4498.


Cite as: https://hdl.handle.net/21.11116/0000-0003-A3B8-5
Abstract
A fluorescent derivative of phalloidin has been synthesized possessing high affinity to filamentous actin. This compound was used for visualization of actin-containing structures in eukaryotic nonmuscle cells. Due to its low molecular weight (1250), fixation for formaldehyde was sufficient to render the membrane permeable for the labeled peptide. Bundles of microfilaments are the predominant pattern in the flat rat kangaroo PtK1 cells, whereas a net of concentric fibers characterizes the more spherical bovine kidney MDBK cells. Specificity of staining was confirmed by competition experiments with unlabeled phalloidin.