English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Analysis of Runx1 Using Induced Gene Ablation Reveals Its Essential Role in Pre-liver HSC Development and Limitations of an In Vivo Approach.

Senserrich, J., Batsivari, A., Rybtsov, S., Gordon-Keylock, S., Souilhol, C., Buchholz, F., et al. (2018). Analysis of Runx1 Using Induced Gene Ablation Reveals Its Essential Role in Pre-liver HSC Development and Limitations of an In Vivo Approach. Stem cell reports, 11(3), 784-794. doi:10.1016/j.stemcr.2018.08.004.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Senserrich, Jordi, Author
Batsivari, Antoniana, Author
Rybtsov, Stanislav, Author
Gordon-Keylock, Sabrina, Author
Souilhol, Celine, Author
Buchholz, Frank1, Author           
Hills, David, Author
Zhao, Suling, Author
Medvinsky, Alexander, Author
Affiliations:
1Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society, ou_2340692              

Content

show
hide
Free keywords: -
 Abstract: Hematopoietic stem cells (HSCs) develop in the embryonic aorta-gonad-mesonephros (AGM) region and subsequently relocate to fetal liver. Runx1 transcription factor is essential for HSC development, but is largely dispensable for adult HSCs. Here, we studied tamoxifen-inducible Runx1 inactivation in vivo. Induction at pre-liver stages (up to embryonic day 10.5) reduced erythromyeloid progenitor numbers, but surprisingly did not block the appearance of Runx1-null HSCs in liver. By contrast, ex vivo analysis showed an absolute Runx1 dependency of HSC development in the AGM region. We found that, contrary to current beliefs, significant Cre-inducing tamoxifen activity persists in mouse blood for at least 72 hr after injection. This deferred recombination can hit healthy HSCs, which escaped early Runx1 ablation and result in appearance of Runx1-null HSCs in liver. Such extended recombination activity in vivo is a potential source of misinterpretation, particularly in analysis of dynamic developmental processes during embryogenesis.

Details

show
hide
Language(s):
 Dates: 2018-09-11
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1016/j.stemcr.2018.08.004
Other: cbg-7268
PMID: 30208304
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Stem cell reports
  Other : Stem Cell Reports
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 11 (3) Sequence Number: - Start / End Page: 784 - 794 Identifier: -