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  Binding of IFT22 to the intraflagellar transport complex is essential for flagellum assembly

Wachter, S., Jung, J., Shafiq, S., Basquin, J., Fort, C., Bastin, P., et al. (2019). Binding of IFT22 to the intraflagellar transport complex is essential for flagellum assembly. EMBO Journal, 38(9): e101251. doi:10.15252/embj.2018101251.

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 Creators:
Wachter, Stefanie1, Author              
Jung, Jamin2, Author
Shafiq, Shahaan2, Author
Basquin, Jerome1, Author              
Fort, Cecile2, Author
Bastin, Philippe2, Author
Lorentzen, Esben2, Author
Affiliations:
1Conti, Elena / Structural Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565144              
2external, ou_persistent22              

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Free keywords: B CORE COMPLEX; TRYPANOSOMA-BRUCEI; CYTOPLASMIC DYNEIN; STRUCTURAL BASIS; PROTEIN; GTPASE; MOUSE; PARTICLES; MOTILITY; HOMOLOGBiochemistry & Molecular Biology; Cell Biology; cilia; GTPase; IFT22; intraflagellar transport; Trypanosoma brucei;
 Abstract: Intraflagellar transport (IFT) relies on motor proteins and the IFT complex to construct cilia and flagella. The IFT complex subunit IFT22/RabL5 has sequence similarity with small GTPases although the nucleotide specificity is unclear because of non-conserved G4/G5 motifs. We show that IFT22 specifically associates with G-nucleotides and present crystal structures of IFT22 in complex with GDP, GTP, and with IFT74/81. Our structural analysis unravels an unusual GTP/GDP-binding mode of IFT22 bypassing the classical G4 motif. The GTPase switch regions of IFT22 become ordered upon complex formation with IFT74/81 and mediate most of the IFT22-74/81 interactions. Structure-based mutagenesis reveals that association of IFT22 with the IFT complex is essential for flagellum construction in Trypanosoma brucei although IFT22 GTP-loading is not strictly required.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published online
 Pages: 18
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000468399700006
DOI: 10.15252/embj.2018101251
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Title: EMBO Journal
  Other : EMBO J.
Source Genre: Journal
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Publ. Info: Nature Publishing Group
Pages: - Volume / Issue: 38 (9) Sequence Number: e101251 Start / End Page: - Identifier: ISSN: 0261-4189
CoNE: https://pure.mpg.de/cone/journals/resource/954925497061