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  Quantification of blumenol derivatives as leaf biomarkers for plant-AMF association

Mindt, E., Wang, M., Schäfer, M., Halitschke, R., & Baldwin, I. T. (2019). Quantification of blumenol derivatives as leaf biomarkers for plant-AMF association. Bio-protocol, 9(14): e3301. doi:10.21769/BioProtoc.3301.

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ITB611.pdf (Publisher version), 210KB
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http://dx.doi.org/10.21769/BioProtoc.3301 (Publisher version)
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 Creators:
Mindt, Elisabeth1, Author              
Wang, Ming1, 2, Author              
Schäfer, Martin1, Author              
Halitschke, Rayko1, Author              
Baldwin, Ian Thomas1, Author              
Affiliations:
1Department of Molecular Ecology, Prof. I. T. Baldwin, MPI for Chemical Ecology, Max Planck Society, ou_24029              
2IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society, Jena, DE, ou_421900              

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 Abstract: Symbiotic interactions between arbuscular mycorrhizal fungi (AMF) and plants are widespread among land plants and can be beneficial for both partners. The plant is provided with mineral nutrients such as nitrogen and phosphorous, whereas it provides carbon resources for the fungus in return. Due to the large economic and environmental impact, efficient characterization methods are required to monitor and quantify plant-AMF colonization. Existing methods, based on destructive sampling and elaborate root tissue analysis, are of limited value for high-throughput (HTP) screening. Here we describe a detailed protocol for the HTP quantification of blumenol derivatives in leaves by a simple extraction procedure and sensitive liquid chromatography mass spectrometry (LC/MS) analysis as accurate proxies of root AMF-associations in both model plants and economically relevant crops.

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 Dates: 20192019-07-20
 Publication Status: Published online
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 Identifiers: Other: ITB611
DOI: 10.21769/BioProtoc.3301
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Title: Bio-protocol
Source Genre: Journal
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Publ. Info: Berkley : Bio Online
Pages: - Volume / Issue: 9 (14) Sequence Number: e3301 Start / End Page: - Identifier: ISSN: 2331-8325
CoNE: https://pure.mpg.de/cone/journals/resource/2331-8325