English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Quantification of blumenol derivatives as leaf biomarkers for plant-AMF association

Mindt, E., Wang, M., Schäfer, M., Halitschke, R., & Baldwin, I. T. (2019). Quantification of blumenol derivatives as leaf biomarkers for plant-AMF association. Bio-protocol, 9(14): e3301. doi:10.21769/BioProtoc.3301.

Item is

Files

show Files
hide Files
:
ITB611.pdf (Publisher version), 210KB
Name:
ITB611.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-

Locators

show
hide
Locator:
http://dx.doi.org/10.21769/BioProtoc.3301 (Publisher version)
Description:
OA
OA-Status:

Creators

show
hide
 Creators:
Mindt, Elisabeth1, Author           
Wang, Ming1, 2, Author           
Schäfer, Martin1, Author           
Halitschke, Rayko1, Author           
Baldwin, Ian Thomas1, Author           
Affiliations:
1Department of Molecular Ecology, Prof. I. T. Baldwin, MPI for Chemical Ecology, Max Planck Society, ou_24029              
2IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society, Jena, DE, ou_421900              

Content

show
hide
Free keywords: -
 Abstract: Symbiotic interactions between arbuscular mycorrhizal fungi (AMF) and plants are widespread among land plants and can be beneficial for both partners. The plant is provided with mineral nutrients such as nitrogen and phosphorous, whereas it provides carbon resources for the fungus in return. Due to the large economic and environmental impact, efficient characterization methods are required to monitor and quantify plant-AMF colonization. Existing methods, based on destructive sampling and elaborate root tissue analysis, are of limited value for high-throughput (HTP) screening. Here we describe a detailed protocol for the HTP quantification of blumenol derivatives in leaves by a simple extraction procedure and sensitive liquid chromatography mass spectrometry (LC/MS) analysis as accurate proxies of root AMF-associations in both model plants and economically relevant crops.

Details

show
hide
Language(s):
 Dates: 20192019-07-20
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: ITB611
DOI: 10.21769/BioProtoc.3301
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Bio-protocol
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Berkley : Bio Online
Pages: - Volume / Issue: 9 (14) Sequence Number: e3301 Start / End Page: - Identifier: ISSN: 2331-8325
CoNE: https://pure.mpg.de/cone/journals/resource/2331-8325