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  Improved sensitivity of whole-cell hybridization by the combination of horseradish peroxidase-labeled oligonucleotides and tyramide signal amplification

Schönhuber, W., Fuchs, B. M., Juretschko, S., & Amann, R. I. (1997). Improved sensitivity of whole-cell hybridization by the combination of horseradish peroxidase-labeled oligonucleotides and tyramide signal amplification. Applied and Environmental Microbiology, 63(8), 3268-3273.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0005-0AFA-7 Version Permalink: https://hdl.handle.net/21.11116/0000-0005-0AFB-6
Genre: Journal Article

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 Creators:
Schönhuber, Wilhelm1, Author           
Fuchs, Bernhard M.1, Author           
Juretschko, S, Author
Amann, Rudolf I.1, Author           
Affiliations:
1Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481696              

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 Abstract: The substrate fluorescein-tyramide was combined with oligonucleotide probes directly labeled with horseradish peroxidase to improve the sensitivity of in situ hybridization of whole fixed bacterial cells. Flow cytometry and quantitative microscopy of cells hybridized by this technique showed 10- to 20-fold signal amplifications relative to fluorescein-manolabeled probes. The application of the new technique to the detection of natural bacterial communities resulted in very bright signals; however, the number of detected cells was significantly lower than that detected with fluorescently monolabeled, rRNA-targeted oligonucleotide probes.

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Language(s): eng - English
 Dates: 1997
 Publication Status: Issued
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: A1997XP06700047
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Title: Applied and Environmental Microbiology
  Other : Appl. Environ. Microbiol.
Source Genre: Journal
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Publ. Info: American Society for Microbiology (ASM)
Pages: - Volume / Issue: 63 (8) Sequence Number: - Start / End Page: 3268 - 3273 Identifier: ISSN: 0099-2240
CoNE: https://pure.mpg.de/cone/journals/resource/954927519600