English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
 Local TagsRelease HistoryDetailsSummary
  Improved sensitivity of whole-cell hybridization by the combination of horseradish peroxidase-labeled oligonucleotides and tyramide signal amplification

Schönhuber, W., Fuchs, B. M., Juretschko, S., & Amann, R. I. (1997). Improved sensitivity of whole-cell hybridization by the combination of horseradish peroxidase-labeled oligonucleotides and tyramide signal amplification. Applied and Environmental Microbiology, 63(8), 3268-3273.

Item is

 

show all hide all

Basic

show hide
Item Permalink: https://hdl.handle.net/21.11116/0000-0005-0AFA-7 Version Permalink: https://hdl.handle.net/21.11116/0000-0005-0AFB-6
Genre: Journal Article

Files

show Files
hide Files
:
Schoenhuber_1997.pdf (Publisher version), 2MB
View   Save
File Permalink:
https://hdl.handle.net/21.11116/0000-0005-0AFC-5
Name:
Schoenhuber_1997.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
View
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Schönhuber, Wilhelm1, Author           
Fuchs, Bernhard M.1, Author           
Juretschko, S, Author
Amann, Rudolf I.1, Author           
Affiliations:
1Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481696              

Content

show
hide
Free keywords: -
 Abstract: The substrate fluorescein-tyramide was combined with oligonucleotide probes directly labeled with horseradish peroxidase to improve the sensitivity of in situ hybridization of whole fixed bacterial cells. Flow cytometry and quantitative microscopy of cells hybridized by this technique showed 10- to 20-fold signal amplifications relative to fluorescein-manolabeled probes. The application of the new technique to the detection of natural bacterial communities resulted in very bright signals; however, the number of detected cells was significantly lower than that detected with fluorescently monolabeled, rRNA-targeted oligonucleotide probes.

Details

show
hide
Language(s): eng - English
 Dates: 1997
 Publication Status: Issued
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: A1997XP06700047
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Applied and Environmental Microbiology
  Other : Appl. Environ. Microbiol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: American Society for Microbiology (ASM)
Pages: - Volume / Issue: 63 (8) Sequence Number: - Start / End Page: 3268 - 3273 Identifier: ISSN: 0099-2240
CoNE: https://pure.mpg.de/cone/journals/resource/954927519600