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  Multi-scale imaging and analysis identify pan-embryo cell dynamics of germlayer formation in zebrafish

Shah, G., Thierbach, K., Schmid, B., Waschke, J., Reade, A., Hlawitschka, M., et al. (2019). Multi-scale imaging and analysis identify pan-embryo cell dynamics of germlayer formation in zebrafish. Nature Communications, 10: 5753. doi:10.1038/s41467-019-13625-0.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0005-638F-B Version Permalink: https://hdl.handle.net/21.11116/0000-0005-7AD4-3
Genre: Journal Article

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 Creators:
Shah, Gopi1, 2, Author
Thierbach, Konstantin3, 4, Author           
Schmid, Benjamin1, 5, Author
Waschke, Johannes6, 7, Author           
Reade, Anna8, 9, Author
Hlawitschka, Mario7, Author
Roeder, Ingo3, Author
Scherf, Nico4, Author           
Huisken, Jan1, 10, Author
Affiliations:
1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany, ou_persistent22              
2European Molecular Biology Laboratory (EMBL), Barcelona, Spain, ou_persistent22              
3Institute for Medical Informatics and Biometry, University Hospital Carl Gustav Carus, Dresden, Germany, ou_persistent22              
4Department Neurophysics (Weiskopf), MPI for Human Cognitive and Brain Sciences, Max Planck Society, ou_2205649              
5Optical Imaging Centre Erlangen (OICE), Friedrich Alexander University Erlangen, Germany, ou_persistent22              
6Department Neurology, MPI for Human Cognitive and Brain Sciences, Max Planck Society, ou_634549              
7Faculty of Computer Science and Media, University of Applied Sciences, Germany, ou_persistent22              
8Cardiovascular Research Institute (CVRI), University of California, San Francisco, CA, USA, ou_persistent22              
9Department of Biochemistry and Biophysics, University of California, San Francisco, CA, USA, ou_persistent22              
10Morgridge Institute for Research, Madison, WI, USA, ou_persistent22              

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Free keywords: Gastrulation; Light-sheet microscopy
 Abstract: The coordination of cell movements across spatio-temporal scales ensures precise positioning of organs during vertebrate gastrulation. Mechanisms governing such morphogenetic movements have been studied only within a local region, a single germlayer or in whole embryos without cell identity. Scale-bridging imaging and automated analysis of cell dynamics are needed for a deeper understanding of tissue formation during gastrulation. Here, we report pan-embryo analyses of formation and dynamics of all three germlayers simultaneously within a developing zebrafish embryo. We show that a distinct distribution of cells in each germlayer is established during early gastrulation via cell movement characteristics that are predominantly determined by their position in the embryo. The differences in initial germlayer distributions are subsequently amplified by a global movement, which organizes the organ precursors along the embryonic body axis, giving rise to the blueprint of organ formation. The tools and data are available as a resource for the community.

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Language(s): eng - English
 Dates: 2018-11-062019-11-172019-12-17
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41467-019-13625-0
PMID: 31848345
PMC: PMC6917746
 Degree: -

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Project name : -
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Funding organization : Max Planck Society
Project name : Smart Multimodal Microscopy for High-Throughput Developmental Biology in Real-Time / SmartMic
Grant ID : 647885
Funding program : Horizon 2020
Funding organization : European Commission (EC)
Project name : -
Grant ID : -
Funding program : -
Funding organization : Morgridge Institute for Research
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Funding program : -
Funding organization : International Max Planck Research School on Neuroscience of Communication: Function, Structure, and Plasticity (IMPRS NeuroCom)
Project name : -
Grant ID : -
Funding program : Institutional Strategy, measure “support the best”
Funding organization : German Federal and State Governments

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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
 Creator(s):
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 10 Sequence Number: 5753 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723