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  MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells.

Gwosch, K., Pape, J. K., Balzarotti, F., Hoess, P., Ellenberg, J., Ries, J., et al. (2020). MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells. Nature Methods, (in press). doi:10.1038/s41592-019-0688-0.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-7AF2-1 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-7AFA-9
Genre: Journal Article

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 Creators:
Gwosch, K.1, Author              
Pape, J. K.1, Author              
Balzarotti, F.1, Author              
Hoess, P., Author
Ellenberg, J., Author
Ries, J., Author
Hell, S. W.1, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              

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 Abstract: The ultimate goal of biological super-resolution fluorescence microscopy is to provide three-dimensional resolution at the size scale of a fluorescent marker. Here we show that by localizing individual switchable fluorophores with a probing donut-shaped excitation beam, MINFLUX nanoscopy can provide resolutions in the range of 1 to 3 nm for structures in fixed and living cells. This progress has been facilitated by approaching each fluorophore iteratively with the probing-donut minimum, making the resolution essentially uniform and isotropic over scalable fields of view. MINFLUX imaging of nuclear pore complexes of a mammalian cell shows that this true nanometer-scale resolution is obtained in three dimensions and in two color channels. Relying on fewer detected photons than standard camera-based localization, MINFLUX nanoscopy is poised to open a new chapter in the imaging of protein complexes and distributions in fixed and living cells.

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Language(s): eng - English
 Dates: 2020-01-20
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/s41592-019-0688-0
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Title: Nature Methods
Source Genre: Journal
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