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  H+ transport by K+ EXCHANGE ANTIPORTER3 promotes photosynthesis and growth in chloroplast ATP synthase mutants

Correa Galvis, V., Strand, D., Messer, M., Thiele, W., Bethmann, S., Hübner, D., et al. (2020). H+ transport by K+ EXCHANGE ANTIPORTER3 promotes photosynthesis and growth in chloroplast ATP synthase mutants. Plant Physiology, 182, 2126-2142. doi:10.1104/pp.19.01561.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0006-0FF4-7 Version Permalink: http://hdl.handle.net/21.11116/0000-0006-9DF9-1
Genre: Journal Article

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Correa Galvis, V.1, Author              
Strand, D.2, Author              
Messer, M.1, Author              
Thiele, W.2, Author              
Bethmann, Stephanie3, Author
Hübner, D.1, Author              
Uflewski, M.1, Author              
Kaiser, E.1, Author              
Siemiatkowska, B.1, Author              
Morris, B. A.1, Author              
Toth, Szilvia Z.3, Author
Watanabe, M.4, Author              
Brückner, F.4, Author              
Hoefgen, R.4, Author              
Jahns, Peter3, Author
Schöttler, M. A.5, Author              
Armbruster, U.1, Author              
Affiliations:
1Regulation of Photosynthesis, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_2205653              
2Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753326              
3external, ou_persistent22              
4Amino Acid and Sulfur Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753337              
5Photosynthesis Research, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753323              

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 Abstract: The composition of the thylakoid proton motive force (pmf) is regulated by thylakoid ion transport. Passive ion channels in the thylakoid membrane dissipate the membrane potential (Δψ) component to allow for a higher fraction of pmf stored as a proton concentration gradient (ΔpH). K+/H+ antiport across the thylakoid membrane via K+ EXCHANGE ANTIPORTER3 (KEA3) instead reduces the ΔpH fraction of the pmf. Thereby, KEA3 decreases non-photochemical quenching (NPQ), thus allowing for higher light use efficiency, which is particularly important during transitions from high to low light. Here, we show that in the background of the Arabidopsis thaliana chloroplast (cp)ATP synthase assembly mutant cgl160, with decreased cpATP synthase activity and increased pmf amplitude, KEA3 plays an important role for photosynthesis and plant growth under steady state conditions. By comparing cgl160 single with cgl160 kea3 double mutants, we demonstrate that in the cgl160 background loss of KEA3 causes a strong growth penalty. This is due to a decreased photosynthetic capacity of cgl160 kea3 mutants, as these plants have a lower lumenal pH than cgl160 mutants, and thus show substantially increased pH-dependent NPQ and decreased electron transport through the cytochrome b6f complex. Overexpression of KEA3 in the cgl160 background decreases pH-dependent NPQ and increases photosystem II efficiency. Taken together, our data provide evidence that under conditions where cpATP synthase activity is low, a KEA3-dependent reduction of ΔpH benefits photosynthesis and growth.

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Language(s): eng - English
 Dates: 2020-01
 Publication Status: Published in print
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Title: Plant Physiology
  Other : Plant Physiol.
Source Genre: Journal
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Publ. Info: Bethesda, Md. : American Society of Plant Biologists
Pages: pp.01561.2019 Volume / Issue: 182 Sequence Number: - Start / End Page: 2126 - 2142 Identifier: ISSN: 0032-0889
CoNE: https://pure.mpg.de/cone/journals/resource/991042744294438