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  Trapped ion mobility spectrometry and PASEF enable in-depth lipidomics from minimal sample amounts

Vasilopoulou, C. G., Sulek, K., Brunner, A.-D., Meitei, N. S., Schweiger-Hufnagel, U., Meyer, S. W., et al. (2020). Trapped ion mobility spectrometry and PASEF enable in-depth lipidomics from minimal sample amounts. NATURE COMMUNICATIONS, 11(1): 331. doi:10.1038/s41467-019-14044-x.

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 Creators:
Vasilopoulou, Catherine G.1, Author              
Sulek, Karolina2, Author
Brunner, Andreas-David1, Author              
Meitei, Ningombam Sanjib2, Author
Schweiger-Hufnagel, Ulrike2, Author
Meyer, Sven W.2, Author
Barsch, Aiko2, Author
Mann, Matthias1, Author              
Meier, Florian1, Author              
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1Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              
2external, ou_persistent22              

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Free keywords: COLLISION CROSS-SECTION; MASS-SPECTROMETRY; COMPREHENSIVE ANALYSIS; LIPIDS; FUNDAMENTALS; PLATFORM
 Abstract: A comprehensive characterization of the lipidome from limited starting material remains very challenging. Here we report a high-sensitivity lipidomics workflow based on nanoflow liquid chromatography and trapped ion mobility spectrometry (TIMS). Taking advantage of parallel accumulation-serial fragmentation (PASEF), we fragment on average 15 precursors in each of 100 ms TIMS scans, while maintaining the full mobility resolution of co-eluting isomers. The acquisition speed of over 100 Hz allows us to obtain MS/MS spectra of the vast majority of isotope patterns. Analyzing 1 mu L of human plasma, PASEF increases the number of identified lipids more than three times over standard TIMS-MS/MS, achieving attomole sensitivity. Building on high intra- and inter-laboratory precision and accuracy of TIMS collisional cross sections (CCS), we compile 1856 lipid CCS values from plasma, liver and cancer cells. Our study establishes PASEF in lipid analysis and paves the way for sensitive, ion mobility-enhanced lipidomics in four dimensions.

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Language(s): eng - English
 Dates: 2020
 Publication Status: Published online
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
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Title: NATURE COMMUNICATIONS
Source Genre: Journal
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Publ. Info: MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND : NATURE PUBLISHING GROUP
Pages: - Volume / Issue: 11 (1) Sequence Number: 331 Start / End Page: - Identifier: ISSN: 2041-1723