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キーワード:
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要旨:
The problems of extending the deoxyglucose method for insects to electron microscopical resolution are discussed. It is calculated that several curies of 3H-deoxyglucose per kilogram body weight may be needed for identification of activity in ultra-thin sections. Oral application may be superior to injection. Incorporation of experimental and control stimulus in the same animal seems appropriate. Freeze-drying may turn out to be the best method of preparing the tissue for plastic embedding. The problem of cutting on a water surface is discussed. It is demonstrated that freeze-drying of Drosophila results in structural preservation sufficient for cell identification in the cervical connective. Autoradiography of ultra-thin sections has not yet been carried out.
