English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  CTCF is dispensable for immune cell transdifferentiation but facilitates an acute inflammatory response

Stik, G., Vidal, E., Barrero, M., Cuartero, S., Vila-Casadesús, M., Mendieta-Esteban, J., et al. (2020). CTCF is dispensable for immune cell transdifferentiation but facilitates an acute inflammatory response. Nature Genetics, In Press. doi:10.1038/s41588-020-0643-0.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0006-8B84-8 Version Permalink: http://hdl.handle.net/21.11116/0000-0006-8C01-B
Genre: Journal Article

Files

show Files
hide Files
:
3237616.pdf (Publisher version), 6MB
 
File Permalink:
-
Name:
3237616.pdf
Description:
-
Visibility:
Restricted ( Max Planck Society (every institute); )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Stik, G., Author
Vidal, E., Author
Barrero, M., Author
Cuartero, S., Author
Vila-Casadesús, M., Author
Mendieta-Esteban, J., Author
Tian, T. V., Author
Choi, J.1, Author              
Berenguer, C., Author
Abad, A., Author
Borsari, B., Author
le Dily, F., Author
Cramer, P.1, Author              
Marti-Renom, M. A., Author
Stadhouders, R., Author
Graf, T., Author
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

Content

show
hide
Free keywords: Epigenomics; Functional genomics; Gene regulation; Immunology
 Abstract: Lettershttps://doi.org/10.1038/s41588-020-0643-01Centre for Genomic Regulation (CRG) and Institute of Science and Technology (BIST), Barcelona, Spain. 2Josep Carreras Leukaemia Research Institute (IJC), Barcelona, Spain. 3CNAG-CRG, Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology (BIST), Barcelona, Spain. 4Vall d’Hebron Institute of Oncology (VHIO), Barcelona, Spain. 5Max Planck Institute for Biophysical Chemistry, Göttingen, Germany. 6ICREA, Barcelona, Spain. 7Department of Pulmonary Medicine, Erasmus MC, Rotterdam, the Netherlands. 8Department of Cell Biology, Erasmus MC, Rotterdam, the Netherlands. 9Universitat Pompeu Fabra (UPF), Barcelona, Spain. ✉e-mail: gregoire.stik@crg.eu; r.stadhouders@erasmusmc.nl; thomas.graf@crg.euThree-dimensional organization of the genome is important for transcriptional regulation1–7. In mammals, CTCF and the cohesin complex create submegabase structures with ele-vated internal chromatin contact frequencies, called topo-logically associating domains (TADs)8–12. Although TADs can contribute to transcriptional regulation, ablation of TAD organization by disrupting CTCF or the cohesin com-plex causes modest gene expression changes13–16. In con-trast, CTCF is required for cell cycle regulation17, embryonic development and formation of various adult cell types18. To uncouple the role of CTCF in cell-state transitions and cell pro-liferation, we studied the effect of CTCF depletion during the conversion of human leukemic B cells into macrophages with minimal cell division. CTCF depletion disrupts TAD orga-nization but not cell transdifferentiation. In contrast, CTCF depletion in induced macrophages impairs the full-blown upregulation of inflammatory genes after exposure to endo-toxin. Our results demonstrate that CTCF-dependent genome topology is not strictly required for a functional cell-fate con-version but facilitates a rapid and efficient response to an external stimulus.

Details

show
hide
Language(s): eng - English
 Dates: 2020-06-08
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1038/s41588-020-0643-0
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature Genetics
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: - Sequence Number: In Press Start / End Page: - Identifier: -