English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  An encryption-decryption framework for validating single-particle imaging

Shen, Z., Teo, C. Z. W., Ayyer, K., & Loh, N. D. (2021). An encryption-decryption framework for validating single-particle imaging. Scientific Reports, 11: 971. doi:10.1038/s41598-020-79589-0.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
s41598-020-79589-0.pdf (Publisher version), 3MB
Name:
s41598-020-79589-0.pdf
Description:
Open Access. - This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
2021
Copyright Info:
© the Author(s)
:
41598_2020_79589_MOESM1_ESM.pdf (Supplementary material), 140KB
Name:
41598_2020_79589_MOESM1_ESM.pdf
Description:
Supplementary Information
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Locator:
https://arxiv.org/abs/2007.03054 (Preprint)
Description:
-
Locator:
https://dx.doi.org/10.1038/s41598-020-79589-0 (Publisher version)
Description:
-

Creators

show
hide
 Creators:
Shen, Z.1, 2, Author
Teo, C. Z. W.1, 2, Author
Ayyer, K.3, 4, Author              
Loh, N. D.1, 2, 5, Author
Affiliations:
1Centre for Bio-imaging Sciences, National University of Singapore, ou_persistent22              
2Department of Physics, National University of Singapore, ou_persistent22              
3Computational Nanoscale Imaging, Condensed Matter Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_3012829              
4Center for Free-Electron Laser Science, ou_persistent22              
5Department of Biological Sciences, National University of Singapore, ou_persistent22              

Content

show
hide
Free keywords: -
 Abstract: We propose an encryption–decryption framework for validating diffraction intensity volumes reconstructed using single-particle imaging (SPI) with X-ray free-electron lasers (XFELs) when the ground truth volume is absent. This conceptual framework exploits each reconstructed volumes’ ability to decipher latent variables (e.g. orientations) of unseen sentinel diffraction patterns. Using this framework, we quantify novel measures of orientation disconcurrence, inconsistency, and disagreement between the decryptions by two independently reconstructed volumes. We also study how these measures can be used to define data sufficiency and its relation to spatial resolution, and the practical consequences of focusing XFEL pulses to smaller foci. This conceptual framework overcomes critical ambiguities in using Fourier Shell Correlation (FSC) as a validation measure for SPI. Finally, we show how this encryption-decryption framework naturally leads to an information-theoretic reformulation of the resolving power of XFEL-SPI, which we hope will lead to principled frameworks for experiment and instrument design.

Details

show
hide
Language(s): eng - English
 Dates: 2020-08-282020-11-172021-01-13
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: arXiv: 2007.03054
DOI: 10.1038/s41598-020-79589-0
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Scientific Reports
  Abbreviation : Sci. Rep.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: London, UK : Nature Publishing Group
Pages: - Volume / Issue: 11 Sequence Number: 971 Start / End Page: - Identifier: ISSN: 2045-2322
CoNE: https://pure.mpg.de/cone/journals/resource/2045-2322