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  Voltage and pH difference across the membrane control the S4 voltage-sensor motion of the Hv1 proton channel

Schladt, T. M., & Berger, T. K. (2020). Voltage and pH difference across the membrane control the S4 voltage-sensor motion of the Hv1 proton channel. Scientific Reports, 10: 21293. doi:10.1038/s41598-020-77986-z.

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s41598-020-77986-z.pdf (Publisher version), 4MB
 
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Schladt, T Moritz1, Author
Berger, Thomas K1, Author
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1Department of Molecular Sensory Systems, Center of Advanced European Studies and Research (caesar), Max Planck Society, Ludwig-Erhard-Allee 2, 53175 Bonn, DE, ou_2173679              

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 Abstract: The voltage-gated proton channel Hv1 is expressed in a variety of cells, including macrophages, sperm, and lung epithelial cells. Hv1 is gated by both the membrane potential and the diference between the intra- and extracellular pH (ΔpH). The coupling of voltage- and ∆pH-sensing is such that Hv1 opens only when the electrochemical proton gradient is outwardly directed. However, the molecular mechanism of this coupling is not known. Here, we investigate the coupling between voltage- and ΔpH-sensing of Ciona intestinalis proton channel (ciHv1) using patch-clamp fuorometry (PCF) and proton uncaging. We show that changes in ΔpH can induce conformational changes of the S4 voltage sensor. Our results are consistent with the idea that S4 can detect both voltage and ΔpH.

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Language(s): eng - English
 Dates: 2020
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: ISI: 33277511
DOI: 10.1038/s41598-020-77986-z
PMID: 33277511
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Title: Scientific Reports
  Abbreviation : Sci Rep
Source Genre: Journal
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Publ. Info: London, UK : Nature Publishing Group
Pages: - Volume / Issue: 10 Sequence Number: 21293 Start / End Page: - Identifier: ISSN: 2045-2322
CoNE: https://pure.mpg.de/cone/journals/resource/2045-2322