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  Mouse embryonic stem cells self-organize into trunk-like structures with neural tube and somites

Veenvliet, J. V., Bolondi, A., Kretzmer, H., Haut, L., Scholze-Wittler, M., Schifferl, D., et al. (2020). Mouse embryonic stem cells self-organize into trunk-like structures with neural tube and somites. Science, 370(6522): eaba4937. doi:10.1126/science.aba4937.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0007-AB69-3 Version Permalink: http://hdl.handle.net/21.11116/0000-0007-AB6A-2
Genre: Journal Article

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© 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science.
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 Creators:
Veenvliet, Jesse V.1, Author              
Bolondi, Adriano2, Author              
Kretzmer, Helene2, Author              
Haut, Leah1, Author
Scholze-Wittler, Manuela1, Author              
Schifferl, Dennis1, Author              
Koch, Frederic1, Author              
Guignard, Léo , Author
Sampath Kumar, Abhishek2, Author              
Pustet, Milena 1, Author
Heimann, Simon1, Author
Buschow, Rene3, Author              
Wittler, Lars1, Author              
Timmermann, Bernd4, Author              
Meissner, Alexander2, Author              
Herrmann, Bernhard G.1, Author              
Affiliations:
1Dept. of Developmental Genetics (Head: Bernhard G. Herrmann), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433548              
2Dept. of Genome Regulation (Head: Alexander Meissner), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_2379694              
3Microscopy and Cryo-Electron Microscopy (Head: Thorsten Mielke), Scientific Service (Head: Christoph Krukenkamp), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479668              
4Sequencing (Head: Bernd Timmermann), Scientific Service (Head: Christoph Krukenkamp), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479670              

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 Abstract: Post-implantation embryogenesis is a highly dynamic process comprising multiple lineage decisions and morphogenetic changes that are inaccessible to deep analysis in vivo. We found that pluripotent mouse embryonic stem cells (mESCs) form aggregates that upon embedding in an extracellular matrix compound induce the formation of highly organized “trunk-like structures” (TLSs) comprising the neural tube and somites. Comparative single-cell RNA sequencing analysis confirmed that this process is highly analogous to mouse development and follows the same stepwise gene-regulatory program. Tbx6 knockout TLSs developed additional neural tubes mirroring the embryonic mutant phenotype, and chemical modulation could induce excess somite formation. TLSs thus reveal an advanced level of self-organization and provide a powerful platform for investigating post-implantation embryogenesis in a dish.

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Language(s): eng - English
 Dates: 2020-12-11
 Publication Status: Published online
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 Identifiers: DOI: 10.1126/science.aba4937
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Title: Science
  Abbreviation : Science
Source Genre: Journal
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Publ. Info: Washington, D.C. : American Association for the Advancement of Science
Pages: - Volume / Issue: 370 (6522) Sequence Number: eaba4937 Start / End Page: - Identifier: ISSN: 0036-8075
CoNE: https://pure.mpg.de/cone/journals/resource/991042748276600_1