ausblenden:
Schlagwörter:
Animals
Male
Mice
Mice, Inbred C57BL
Mice, Mutant Strains
Neuropil/*metabolism
Polyribosomes/metabolism
*Protein Biosynthesis
Proteome/metabolism
RNA, Messenger/genetics/*metabolism
Ribosomes/*metabolism
Synapses/*metabolism
Zusammenfassung:
To accommodate their complex morphology, neurons localize messenger RNAs (mRNAs) and ribosomes near synapses to produce proteins locally. However, a relative paucity of polysomes (considered the active sites of translation) detected in electron micrographs of neuronal processes has suggested a limited capacity for local protein synthesis. In this study, we used polysome profiling together with ribosome footprinting of microdissected rodent synaptic regions to reveal a surprisingly high number of dendritic and/or axonal transcripts preferentially associated with monosomes (single ribosomes). Furthermore, the neuronal monosomes were in the process of active protein synthesis. Most mRNAs showed a similar translational status in the cell bodies and neurites, but some transcripts exhibited differential ribosome occupancy in the compartments. Monosome-preferring transcripts often encoded high-abundance synaptic proteins. Thus, monosome translation contributes to the local neuronal proteome.
