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  Functional expression of bacteriorhodopsin in oocytes allows direct measurement of voltage dependence of light induced H+ pumping

Nagel, G., Möckel, B., Büldt, G., & Bamberg, E. (1995). Functional expression of bacteriorhodopsin in oocytes allows direct measurement of voltage dependence of light induced H+ pumping. FEBS Letters, 377(2), 263-266. doi:10.1016/0014-5793(95)01356-3.

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 Creators:
Nagel, Georg1, Author           
Möckel, Bettina2, Author
Büldt, Georg2, Author
Bamberg, Ernst1, Author           
Affiliations:
1Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2068289              
2IBI-2, Forschungszentrum Jülich, D-52425 Jülich, Germany, ou_persistent22              

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Free keywords: Bacteriorhodopsin; H+-pump; Voltage dependence; Xenopus oocyte
 Abstract: We report on the first successful expression of the light driven H+- pump, bacteriorhodopsin, into the plasma membrane of oocytes from Xenopus laevis. The light induced photocurrents which reflect the pumping of H+- by BR were analysed under voltage clamp conditions. At least 100 active BR molecules per μm2 were expressed in the plasma membrane so that both the voltage clamp and giant patch clamp method could be applied. We show that H+- pumping by BR is modulated by the membrane potential, i.e. the pump current shows strong voltage dependence in the range measured between −165 mV to +60 mV.

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Language(s): eng - English
 Dates: 1995-10-272000-03-091995-12-18
 Publication Status: Issued
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/0014-5793(95)01356-3
 Degree: -

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Title: FEBS Letters
  Other : FEBS Lett.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 377 (2) Sequence Number: - Start / End Page: 263 - 266 Identifier: ISSN: 0014-5793
CoNE: https://pure.mpg.de/cone/journals/resource/954925399501