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  Involvement of the GTP binding protein Rho in constitutive endocytosis in Xenopus laevis oocytes

Schmalzing, G., Richter, H.-P., Hansen, A., Schwarz, W., Just, I., & Aktories, K. (1995). Involvement of the GTP binding protein Rho in constitutive endocytosis in Xenopus laevis oocytes. The Journal of Cell Biology: JCB, 130(6), 1319-1332. doi:10.1083/jcb.130.6.1319.

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Schmalzing, Günther1, Autor           
Richter, Hans-Peter2, Autor
Hansen, Alexander3, Autor
Schwarz, Wolfgang1, Autor           
Just, Ingo3, Autor
Aktories, Klaus3, Autor
Affiliations:
1Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2068289              
2Institut for Physiologie, Universität des Saarlandes, D-66421 Homburg-Saar, ou_persistent22              
3Institut für Pharmakologie und Toxikologie, Universität Freiburg, D-79109 Freiburg, Germany, ou_persistent22              

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 Zusammenfassung: To study an endocytotic role of the GTP-binding protein RhoA in Xenopus oocytes, we have monitored changes in the surface expression of sodium pumps, the surface area of the oocyte and the uptake of the fluid-phase marker inulin. Xenopus oocytes possess intracellular sodium pumps that are continuously exchanged for surface sodium pumps by constitutive endo- and exocytosis. Injection of Clostridium botulinum C3 exoenzyme, which inactivates Rho by ADP-ribosylation, induced a redistribution of virtually all intracellular sodium pumps to the plasma membrane and increased the surface area of the oocytes. The identical effects were caused by injection of ADP-ribosylated recombinant RhoA into oocytes. The C3 exoenzyme acts by blocking constitutive endocytosis in oocytes, as determined using a mAb to the beta 1 subunit of the mouse sodium pump as a reporter molecule and oocytes expressing heterologous sodium pumps. In contrast, an increase in endocytosis and a decrease in the surface area was induced by injection of recombinant Val14-RhoA protein or Val14-rhoA cRNA. PMA stimulated sodium pump endocytosis, an effect that was blocked by a specific inhibitor of protein kinase C (Gö 16) or by ADP-ribosylation of Rho by C3. Similarly, the phorbol ester-induced increase in fluid-phase endocytosis in oocytes was inhibited by Gö 16, C3 transferase, or by injection of ADP-ribosylated RhoA. In contrast to C3 transferase, C. botulinum C2 transferase, which ADP-ribosylates actin, had no effect on sodium pump endocytosis or PMA-stimulated fluid-phase endocytosis. The data suggests that RhoA is an essential component of a presumably clathrin-independent endocytic pathway in Xenopus oocytes which can be regulated by protein kinase C.

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Sprache(n): eng - English
 Datum: 1995-05-151995-01-031995-09-15
 Publikationsstatus: Erschienen
 Seiten: 14
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1083/jcb.130.6.1319
PMID: 7559755
PMC: PMC2120574
 Art des Abschluß: -

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Titel: The Journal of Cell Biology : JCB
  Andere : J. Cell Biol.
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: New York, NY : Rockefeller Institute Press
Seiten: - Band / Heft: 130 (6) Artikelnummer: - Start- / Endseite: 1319 - 1332 Identifikator: ISSN: 0021-9525
CoNE: https://pure.mpg.de/cone/journals/resource/991042742946024