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  Involvement of the GTP binding protein Rho in constitutive endocytosis in Xenopus laevis oocytes

Schmalzing, G., Richter, H.-P., Hansen, A., Schwarz, W., Just, I., & Aktories, K. (1995). Involvement of the GTP binding protein Rho in constitutive endocytosis in Xenopus laevis oocytes. The Journal of Cell Biology: JCB, 130(6), 1319-1332. doi:10.1083/jcb.130.6.1319.

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アイテムのパーマリンク: https://hdl.handle.net/21.11116/0000-0008-2841-2 版のパーマリンク: https://hdl.handle.net/21.11116/0000-0008-2842-1
資料種別: 学術論文

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 作成者:
Schmalzing, Günther1, 著者           
Richter, Hans-Peter2, 著者
Hansen, Alexander3, 著者
Schwarz, Wolfgang1, 著者           
Just, Ingo3, 著者
Aktories, Klaus3, 著者
所属:
1Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2068289              
2Institut for Physiologie, Universität des Saarlandes, D-66421 Homburg-Saar, ou_persistent22              
3Institut für Pharmakologie und Toxikologie, Universität Freiburg, D-79109 Freiburg, Germany, ou_persistent22              

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 要旨: To study an endocytotic role of the GTP-binding protein RhoA in Xenopus oocytes, we have monitored changes in the surface expression of sodium pumps, the surface area of the oocyte and the uptake of the fluid-phase marker inulin. Xenopus oocytes possess intracellular sodium pumps that are continuously exchanged for surface sodium pumps by constitutive endo- and exocytosis. Injection of Clostridium botulinum C3 exoenzyme, which inactivates Rho by ADP-ribosylation, induced a redistribution of virtually all intracellular sodium pumps to the plasma membrane and increased the surface area of the oocytes. The identical effects were caused by injection of ADP-ribosylated recombinant RhoA into oocytes. The C3 exoenzyme acts by blocking constitutive endocytosis in oocytes, as determined using a mAb to the beta 1 subunit of the mouse sodium pump as a reporter molecule and oocytes expressing heterologous sodium pumps. In contrast, an increase in endocytosis and a decrease in the surface area was induced by injection of recombinant Val14-RhoA protein or Val14-rhoA cRNA. PMA stimulated sodium pump endocytosis, an effect that was blocked by a specific inhibitor of protein kinase C (Gö 16) or by ADP-ribosylation of Rho by C3. Similarly, the phorbol ester-induced increase in fluid-phase endocytosis in oocytes was inhibited by Gö 16, C3 transferase, or by injection of ADP-ribosylated RhoA. In contrast to C3 transferase, C. botulinum C2 transferase, which ADP-ribosylates actin, had no effect on sodium pump endocytosis or PMA-stimulated fluid-phase endocytosis. The data suggests that RhoA is an essential component of a presumably clathrin-independent endocytic pathway in Xenopus oocytes which can be regulated by protein kinase C.

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言語: eng - English
 日付: 1995-05-151995-01-031995-09-15
 出版の状態: 出版
 ページ: 14
 出版情報: -
 目次: -
 査読: 査読あり
 識別子(DOI, ISBNなど): DOI: 10.1083/jcb.130.6.1319
PMID: 7559755
PMC: PMC2120574
 学位: -

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出版物 1

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出版物名: The Journal of Cell Biology : JCB
  その他 : J. Cell Biol.
種別: 学術雑誌
 著者・編者:
所属:
出版社, 出版地: New York, NY : Rockefeller Institute Press
ページ: - 巻号: 130 (6) 通巻号: - 開始・終了ページ: 1319 - 1332 識別子(ISBN, ISSN, DOIなど): ISSN: 0021-9525
CoNE: https://pure.mpg.de/cone/journals/resource/991042742946024