English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Distribution of parathyroid hormone-stimulated adenylate cyclase in plasma membranes of cells of the kidney cortex

Shlatz, L. J., Schwartz, I. L., Kinne-Saffran, E., & Kinne, R. (1975). Distribution of parathyroid hormone-stimulated adenylate cyclase in plasma membranes of cells of the kidney cortex. Journal of Membrane Biology, 24(2), 131-144. doi:10.1007/BF01868619.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Shlatz, Linda J.1, 2, Author           
Schwartz, Irving L.1, 2, Author           
Kinne-Saffran, Evamaria1, 2, Author           
Kinne, Rolf1, 2, Author           
Affiliations:
1Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068297              
2Department of Physiology and Biophysics, Mount Sinai Medical and Graduate Schools of the City University of New York, 10029, New York, N.Y.; USA, ou_persistent22              

Content

show
hide
Free keywords: Luminal; Calcitonin; Vasopressin; Parathyroid Hormone; Adenylate Cyclase
 Abstract: Free flow electrophoresis was employed to separate renal cortical plasma membranes into luminal (brush border microvilli) and contraluminal (basal-lateral membrane) fractions. During the separation adenylate cyclase activity was found to parallel the activity of Na+-K+-activated ATPase, an enzyme which is present in contraluminal but not in luminal membranes. In the basal-lateral membrane fraction the specific activities of adenylate cyclase and Na+-K+-activated ATPase were 4.4 and 4.6 times greater, respectively, than in the brush border fraction. The adenylate cyclase of the basal-lateral membrane fraction was specifically stimulated by parathyroid hormone which maximally increased enzyme activity eightfold. The biologically active (1-34) peptide fragment of paratyhroid hormone produced a 350% increase in adenylate cyclase activity. In contrast, calcitonin, epinephrine and vasopressin maximally stimulated the enzyme by only 55, 35 and 30%, respectively. These results indicate that adenylate cyclase, specifically stimulated by parathyroid hormone, is distributed preferentially in the contraluminal region of the plasma membrane of renal cortical epithelial cells.

Details

show
hide
Language(s): eng - English
 Dates: 1975-07-081975-04-241975-11-07
 Publication Status: Issued
 Pages: 14
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/BF01868619
PMID: 172637
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Membrane Biology
  Other : J. Membr. Biol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: New York : Springer-Verlag New York
Pages: - Volume / Issue: 24 (2) Sequence Number: - Start / End Page: 131 - 144 Identifier: ISSN: 0022-2631
CoNE: https://pure.mpg.de/cone/journals/resource/954925415943