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  Rapid multi-plane phase-contrast microscopy reveals torsional dynamics in flagellar motion

Mojiri, S., Isbaner, S., Mühle, S., Jang, H., Bae, A. J., Gregor, I., et al. (2021). Rapid multi-plane phase-contrast microscopy reveals torsional dynamics in flagellar motion. Biomedical Optics Express, 12: 6, pp. 3169. doi:10.1364/BOE.419099.

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 Creators:
Mojiri, Soheil, Author
Isbaner, Sebastian, Author
Mühle, Steffen, Author
Jang, Hongje, Author
Bae, Albert J.1, Author           
Gregor, Ingo, Author
Gholami, Azam1, Author           
Enderlein, Jörg, Author
Affiliations:
1Laboratory for Fluid Physics, Pattern Formation and Biocomplexity, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, ou_2063287              

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 Abstract: High speed volumetric optical microscopy is an important tool for observing rapid processes in living cells or for real-time tracking of sub-cellular components. However, the 3D imaging capability often comes at the price of a high technical complexity of the imaging system and/or the requirement of demanding image analysis. Here, we propose a combination of conventional phase-contrast imaging with a customized multi-plane beam-splitter for enabling simultaneous acquisition of images in eight different focal planes. Our method is technically straightforward and does not require complex post-processing image analysis. We apply our multi-plane phase-contrast microscope to the real-time observation of the fast motion of reactivated Chlamydomonas axonemes with sub-µm spatial and 4 ms temporal resolution. Our system allows us to observe not only bending but also the three-dimensional torsional dynamics of these micro-swimmers.

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Language(s): eng - English
 Dates: 2021-05-072021
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1364/BOE.419099
 Degree: -

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Title: Biomedical Optics Express
Source Genre: Journal
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Pages: - Volume / Issue: 12 Sequence Number: 6 Start / End Page: 3169 Identifier: ISSN: 2156-7085
ISSN: 2156-7085