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Abstract:
Forster resonance energy transfer (FRET) microscopy is a powerful
fluorescence microscopy method to study the nanoscale organization of
multiprotein assemblies in vivo. Moreover, many biochemical and
biophysical processes can be followed by employing sophisticated FRET
biosensors directly in living cells. Here, we summarize existing FRET
experiments and biosensors applied in yeasts Saccharomyces cerevisiae
and Schizosaccharomyces pombe, two important models of fundamental
biomedical research and efficient platforms for analyses of bioactive
molecules. We aim to provide a practical guide on suitable FRET
techniques, fluorescent proteins, and experimental setups available for
successful FRET experiments in yeasts.
