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  Proton gradients from light-harvesting E. coli control DNA assemblies for synthetic cells

Jahnke, K., Ritzmann, N., Fichtler, J., Nitschke, A., Dreher, Y., Abele, T., et al. (2021). Proton gradients from light-harvesting E. coli control DNA assemblies for synthetic cells. Nature Communications, 12: 3967 (2021), pp. 1-9. doi:10.1038/s41467-021-24103-x.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0008-C548-9 Version Permalink: https://hdl.handle.net/21.11116/0000-0008-C549-8
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 Creators:
Jahnke, Kevin1, Author           
Ritzmann, Noah, Author
Fichtler, Julius1, Author           
Nitschke, Anna1, Author           
Dreher, Yannik1, Author           
Abele, Tobias1, Author           
Hofhaus, Götz, Author
Platzman, Ilia1, Author           
Schröder, Rasmus R., Author
Müller, Daniel J., Author
Spatz, Joachim P.1, Author           
Göpfrich, Kerstin, Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

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Free keywords: Biological physics; DNA nanostructures; Light harvesting; Synthetic biology
 Abstract: Bottom-up and top-down approaches to synthetic biology each employ distinct methodologies with the common aim to harness living systems. Here, we realize a strategic merger of both approaches to convert light into proton gradients for the actuation of synthetic cellular systems. We genetically engineer E. coli to overexpress the light-driven inward-directed proton pump xenorhodopsin and encapsulate them in artificial cell-sized compartments. Exposing the compartments to light-dark cycles, we reversibly switch the pH by almost one pH unit and employ these pH gradients to trigger the attachment of DNA structures to the compartment periphery. For this purpose, a DNA triplex motif serves as a nanomechanical switch responding to the pH-trigger of the E. coli. When DNA origami plates are modified with the pH-sensitive triplex motif, the proton-pumping E. coli can trigger their attachment to giant unilamellar lipid vesicles (GUVs) upon illumination. A DNA cortex is formed upon DNA origami polymerization, which sculpts and deforms the GUVs. We foresee that the combination of bottom-up and top down approaches is an efficient way to engineer synthetic cells.

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Language(s): eng - English
 Dates: 2021-01-282021-05-272021-06-25
 Publication Status: Published online
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41467-021-24103-x
 Degree: -

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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 12 Sequence Number: 3967 (2021) Start / End Page: 1 - 9 Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723