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  Mouse multipotent progenitor 5 cells are located at the interphase between hematopoietic stem and progenitor cells

Sommerkamp, P., Romero-Mulero, M. C., Narr, A., Ladel, L., Hustin, L., Schönberger, K., et al. (2021). Mouse multipotent progenitor 5 cells are located at the interphase between hematopoietic stem and progenitor cells. Blood, 137, 3218-3224. doi:10.1182/blood.2020007876.

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 Creators:
Sommerkamp, Pia1, Author
Romero-Mulero, Mari Carmen2, Author
Narr, Andreas1, Author
Ladel, Luisa1, Author
Hustin, Lucie1, Author
Schönberger, Katharina2, Author           
Renders, Simon1, Author
Altamura, Sandro1, Author
Zeisberger, Petra1, Author
Jäcklein, Karin2, Author
Klimmeck, Daniel1, Author
Rodriguez-Fraticelli, Alejo1, Author
Camargo, Fernando D.1, Author
Perié, Leïla1, Author
Trumpp, Andreas1, Author
Cabezas-Wallscheid, Nina2, Author           
Affiliations:
1External Organizations, ou_persistent22              
2Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243641              

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Free keywords: HEMATOPOIESIS; Stem and Primitive Progenitor Cells
 Abstract: Hematopoietic stem cells (HSCs) and distinct multipotent progenitor (MPP) populations (MPP1-4) contained within the LinSca-1+c-Kit+ (LSK) compartment have previously been identified using diverse surface-marker panels. Here, we phenotypically define and functionally characterize MPP5 (LSK CD34+CD135CD48CD150). Upon transplantation, MPP5 supports initial emergency myelopoiesis followed by stable contribution to the lymphoid lineage. MPP5, capable of generating MPP1-4 but not HSCs, represents a dynamic and versatile component of the MPP network. To characterize all hematopoietic stem and progenitor cells, we performed RNA-sequencing (RNA-seq) analysis to identify specific transcriptomic landscapes of HSCs and MPP1-5. This was complemented by single-cell RNA-seq analysis of LSK cells to establish the differentiation trajectories from HSCs to MPP1-5. In agreement with functional reconstitution activity, MPP5 is located immediately downstream of HSCs but upstream of the more committed MPP2-4. This study provides a comprehensive analysis of the LSK compartment, focusing on the functional and molecular characteristics of the newly defined MPP5 subset.

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Language(s): eng - English
 Dates: 2021-06-10
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1182/blood.2020007876
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Title: Blood
  Other : Blood
Source Genre: Journal
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Publ. Info: Philadelphia, Pa. : W.B. Saunders
Pages: - Volume / Issue: 137 Sequence Number: - Start / End Page: 3218 - 3224 Identifier: ISSN: 0006-4971
CoNE: https://pure.mpg.de/cone/journals/resource/954925385125