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キーワード:
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要旨:
A cellulase gene of Clostridium cellobioparum was cloned on vector pUC19 to form a recombinant plasmid pCMC1 and expressed in Escherichia coli. The expressed enzyme showed carboxymethylcellulase activity but could not degrade Avicel. E. coli JM109 carrying pCMC1 excreted a large amount of cellulase into a culture medium. The C. cellobioparum DNA sequence in the pCMC1 directed the cellulase excretion.