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  Photoactivatable fluorescent dyes with hydrophilic caging groups and their use in multicolor nanoscopy

Butkevich, A. N., Weber, M., Cereceda Delgado, A., Ostersehlt, L. M., d'Este, E., & Hell, S. W. (2021). Photoactivatable fluorescent dyes with hydrophilic caging groups and their use in multicolor nanoscopy. Journal of the American Chemical Society, 143(44), 18388-18393. doi:10.1021/jacs.1c09999.

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 Creators:
Butkevich, A. N.1, Author              
Weber, M.1, Author              
Cereceda Delgado, A.R.1, Author              
Ostersehlt, L. M.1, Author              
d'Este, E., Author
Hell, S. W.1, Author              
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1Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              

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 Abstract: We propose a series of fluorescent dyes with hydrophilic carbamate caging groups that undergo rapid photoactivation under UV (≤400 nm) irradiation but do not undergo spurious two-photon activation with high-intensity (visible or infrared) light of about twice the wavelength. The caged fluorescent dyes and labels derived therefrom display high water solubility and convert upon photoactivation into validated super-resolution and live-cell-compatible fluorophores. In combination with popular fluorescent markers, multiple (up to six)-color images can be obtained with stimulated emission depletion nanoscopy. Moreover, individual fluorophores can be localized with precision <3 nm (standard deviation) using MINSTED and MINFLUX techniques.

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Language(s): eng - English
 Dates: 2021-10-292021-11-10
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1021/jacs.1c09999
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Title: Journal of the American Chemical Society
Source Genre: Journal
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Pages: 6 Volume / Issue: 143 (44) Sequence Number: - Start / End Page: 18388 - 18393 Identifier: -