Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
  A 37 kb region upstream of brachyury comprising a notochord enhancer is essential for notochord and tail development

Schifferl, D., Scholze-Wittler, M., Wittler, L., Veenvliet, J. V., Koch, F., & Herrmann, B. G. (2021). A 37 kb region upstream of brachyury comprising a notochord enhancer is essential for notochord and tail development. Development, 148(23): dev200059. doi:10.1242/dev.200059.

Item is

Basisdaten

einblenden: ausblenden:
Genre: Zeitschriftenartikel

Dateien

einblenden: Dateien
ausblenden: Dateien
:
Schifferl et al_2021.pdf (Verlagsversion), 19MB
Name:
Schifferl et al_2021.pdf
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Öffentlich
MIME-Typ / Prüfsumme:
application/pdf / [MD5]
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
© 2021. Published by The Company of Biologists Ltd

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Schifferl, Dennis1, Autor           
Scholze-Wittler, Manuela1, Autor           
Wittler, Lars1, Autor           
Veenvliet, Jesse V.1, Autor           
Koch, Frederic1, Autor           
Herrmann, Bernhard G.1, Autor           
Affiliations:
1Dept. of Developmental Genetics (Head: Bernhard G. Herrmann), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433548              

Inhalt

einblenden:
ausblenden:
Schlagwörter: Mouse, Embryo, Development, Notochord, Brachyury, Enhancer
 Zusammenfassung: The node-streak border region comprising notochord progenitor cells (NPCs) at the posterior node and neuro-mesodermal progenitor cells (NMPs) in the adjacent epiblast is the prime organizing center for axial elongation in mouse embryos. The T-box transcription factor brachyury (T) is essential for both formation of the notochord and maintenance of NMPs, and thus is a key regulator of trunk and tail development. The T promoter controlling T expression in NMPs and nascent mesoderm has been characterized in detail; however, control elements for T expression in the notochord have not been identified yet. We have generated a series of deletion alleles by CRISPR/Cas9 genome editing in mESCs, and analyzed their effects in mutant mouse embryos. We identified a 37 kb region upstream of T that is essential for notochord function and tailbud outgrowth. Within that region, we discovered a T-binding enhancer required for notochord cell specification and differentiation. Our data reveal a complex regulatory landscape controlling cell type-specific expression and function of T in NMP/nascent mesoderm and node/notochord, allowing proper trunk and tail development.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2021-11-082021-12-15
 Publikationsstatus: Online veröffentlicht
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: DOI: 10.1242/dev.200059
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Development
  Andere : Development
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Cambridge, Cambridgeshire : Company of Biologists
Seiten: - Band / Heft: 148 (23) Artikelnummer: dev200059 Start- / Endseite: - Identifikator: ISSN: 0950-1991
CoNE: https://pure.mpg.de/cone/journals/resource/954927546241