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  A 37 kb region upstream of brachyury comprising a notochord enhancer is essential for notochord and tail development

Schifferl, D., Scholze-Wittler, M., Wittler, L., Veenvliet, J. V., Koch, F., & Herrmann, B. G. (2021). A 37 kb region upstream of brachyury comprising a notochord enhancer is essential for notochord and tail development. Development, 148(23): dev200059. doi:10.1242/dev.200059.

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Schifferl et al_2021.pdf (Publisher version), 19MB
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© 2021. Published by The Company of Biologists Ltd

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 Creators:
Schifferl, Dennis1, Author              
Scholze-Wittler, Manuela1, Author              
Wittler, Lars1, Author              
Veenvliet, Jesse V.1, Author              
Koch, Frederic1, Author              
Herrmann, Bernhard G.1, Author              
Affiliations:
1Dept. of Developmental Genetics (Head: Bernhard G. Herrmann), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433548              

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Free keywords: Mouse, Embryo, Development, Notochord, Brachyury, Enhancer
 Abstract: The node-streak border region comprising notochord progenitor cells (NPCs) at the posterior node and neuro-mesodermal progenitor cells (NMPs) in the adjacent epiblast is the prime organizing center for axial elongation in mouse embryos. The T-box transcription factor brachyury (T) is essential for both formation of the notochord and maintenance of NMPs, and thus is a key regulator of trunk and tail development. The T promoter controlling T expression in NMPs and nascent mesoderm has been characterized in detail; however, control elements for T expression in the notochord have not been identified yet. We have generated a series of deletion alleles by CRISPR/Cas9 genome editing in mESCs, and analyzed their effects in mutant mouse embryos. We identified a 37 kb region upstream of T that is essential for notochord function and tailbud outgrowth. Within that region, we discovered a T-binding enhancer required for notochord cell specification and differentiation. Our data reveal a complex regulatory landscape controlling cell type-specific expression and function of T in NMP/nascent mesoderm and node/notochord, allowing proper trunk and tail development.

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Language(s): eng - English
 Dates: 2021-11-082021-12-15
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1242/dev.200059
 Degree: -

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Title: Development
  Other : Development
Source Genre: Journal
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Publ. Info: Cambridge, Cambridgeshire : Company of Biologists
Pages: - Volume / Issue: 148 (23) Sequence Number: dev200059 Start / End Page: - Identifier: ISSN: 0950-1991
CoNE: https://pure.mpg.de/cone/journals/resource/954927546241