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  Live-cell imaging of RNA transport in axons of cultured primary neurons

Hees, J. T., & Harbauer, A. B. (2022). Live-cell imaging of RNA transport in axons of cultured primary neurons. In A. Vagnoni (Ed.), Axonal Transport. Methods and Protocols (1, pp. 225-237). New York: Humana.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000A-533A-8 Version Permalink: https://hdl.handle.net/21.11116/0000-000A-533B-7
Genre: Book Chapter

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 Creators:
Hees, J. Tabitha1, Author           
Harbauer, Angelika B.1, Author           
Affiliations:
1Max Planck Research Group: Neurometabolism / Harbauer, MPI of Neurobiology, Max Planck Society, ou_3232739              

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Free keywords: RNA transport MS2 PP7 Split-Venus Transient transfection Cultured primary neurons Imaging
 Abstract: The use of fluorescent proteins has revolutionized the study of protein localization and transport. However, the visualization of other molecules and specifically RNA during live-cell imaging remains challenging. In this chapter, we provide guidance to the available methods, their advantages and drawbacks as well as provide a detailed protocol for the detection of RNA transport using the MS2/PP7-split-Venus system for background-free RNA imaging.

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 Dates: 2022-04-13
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: MEDLINE:35412279
DOI: 10.1007/978-1-0716-1990-2_11
 Degree: -

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Title: Axonal Transport. Methods and Protocols
Source Genre: Book
 Creator(s):
Vagnoni, Alessio, Editor
Affiliations:
-
Publ. Info: New York : Humana, 1
Pages: 573 Volume / Issue: - Sequence Number: - Start / End Page: 225 - 237 Identifier: ISBN: 978-1-0716-1989-6

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Title: Methods in molecular biology
Source Genre: Series
 Creator(s):
Clifton, N. J., Editor
Affiliations:
-
Publ. Info: -
Pages: - Volume / Issue: 2431 Sequence Number: - Start / End Page: - Identifier: -