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  Bottom-up assembly of synthetic cells with a DNA cytoskeleton

Jahnke, K., Huth, V., Mersdorf, U., Liu, N., & Göpfrich, K. (2022). Bottom-up assembly of synthetic cells with a DNA cytoskeleton. ACS Nano, 16(5), 7233-7241. doi:10.1021/acsnano.1c10703.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000A-8C5B-3 Version Permalink: https://hdl.handle.net/21.11116/0000-000A-8C5C-2
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 Creators:
Jahnke, Kevin1, Author           
Huth, Vanessa1, Author           
Mersdorf, Ulrike2, Author           
Liu, Na, Author
Göpfrich, Kerstin1, Author           
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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Free keywords: DNA nanotechnology, giant unilamellar vesicles, azobenzene, DNA nanotube, synthetic cell, bottom-up synthetic biology
 Abstract: Cytoskeletal elements, like actin and myosin, have been reconstituted inside lipid vesicles toward the vision to reconstruct cells from the bottom up. Here, we realize the de novo assembly of entirely artificial DNA-based cytoskeletons with programmed multifunctionality inside synthetic cells. Giant unilamellar lipid vesicles (GUVs) serve as cell-like compartments, in which the DNA cytoskeletons are repeatedly and reversibly assembled and disassembled with light using the cis–trans isomerization of an azobenzene moiety positioned in the DNA tiles. Importantly, we induced ordered bundling of hundreds of DNA filaments into more rigid structures with molecular crowders. We quantify and tune the persistence length of the bundled filaments to achieve the formation of ring-like cortical structures inside GUVs, resembling actin rings that form during cell division. Additionally, we show that DNA filaments can be programmably linked to the compartment periphery using cholesterol-tagged DNA as a linker. The linker concentration determines the degree of the cortex-like network formation, and we demonstrate that the DNA cortex-like network can deform GUVs from within. All in all, this showcases the potential of DNA nanotechnology to mimic the diverse functions of a cytoskeleton in synthetic cells.

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Language(s): eng - English
 Dates: 2021-12-022022-03-282022-04-042022
 Publication Status: Issued
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1021/acsnano.1c10703
 Degree: -

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Title: ACS Nano
  Other : ACS Nano
Source Genre: Journal
 Creator(s):
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Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: 16 (5) Sequence Number: - Start / End Page: 7233 - 7241 Identifier: ISSN: 1936-0851
CoNE: https://pure.mpg.de/cone/journals/resource/1936-0851