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  Measurement of arbitrary scan patterns for correction of imaging distortions in laser scanning microscopy

Rose, P., Klioutchnikov, A., Wallace, D. J., Greenberg, D. S., Kerr, J. N. D., & Sawinski, J. (2022). Measurement of arbitrary scan patterns for correction of imaging distortions in laser scanning microscopy. Biomedical Optics Express, 13(7), 3983-3992.

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© 2022 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement [...] Creative Commons Licensing: Some authors, as a condition of their funding, must publish their work under a Creative Commons license. We therefore offer a CC BY license for authors who indicate that their work is funded by agencies that we have confirmed have this requirement. Authors must enter their funder(s) during the manuscript submission process. At that point, if appropriate, the CC BY license option will be available to select for an additional fee. Copyright Transfer and Open Access Publishing Agreement: [...] AUTHOR(S) RIGHTS: (c) Preprint Servers. The Author(s) are permitted to post and update the Author Accepted version or Final Publisher Version of Record on preprint servers, e.g. arXiv. Any such posting made after publication of the Work shall include a link to the online abstract in the Journal and the copyright notice below.
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© 2022 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement [...] Creative Commons Licensing: Some authors, as a condition of their funding, must publish their work under a Creative Commons license. We therefore offer a CC BY license for authors who indicate that their work is funded by agencies that we have confirmed have this requirement. Authors must enter their funder(s) during the manuscript submission process. At that point, if appropriate, the CC BY license option will be available to select for an additional fee. Copyright Transfer and Open Access Publishing Agreement: [...] AUTHOR(S) RIGHTS: (c) Preprint Servers. The Author(s) are permitted to post and update the Author Accepted version or Final Publisher Version of Record on preprint servers, e.g. arXiv. Any such posting made after publication of the Work shall include a link to the online abstract in the Journal and the copyright notice below.
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 Creators:
Rose, Patrick1, Author
Klioutchnikov, Alexandr2, Author           
Wallace, Damian J2, Author                 
Greenberg, David S.1, Author
Kerr, Jason N. D.2, Author                 
Sawinski, Jürgen2, Author                 
Affiliations:
1External Organizations, ou_persistent22              
2Department of Behavior and Brain Organization, Max Planck Institute for Neurobiology of Behavior – caesar, Max Planck Society, ou_3361760              

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 Abstract: Laser scanning microscopy requires beam steering through relay and focusing optics at sub-micron precision. In light-weight mobile systems, such as head mounted multiphoton microscopes, distortion and imaging plane curvature management is unpractical due to the complexity of required optic compensation. Thus, the resulting scan pattern limits anatomical fidelity and decreases analysis algorithm efficiency. Here, we present a technique that reconstructs the three-dimensional scan path only requiring translation of a simple fluorescent test probe. Our method is applicable to any type of scanning instrument with sectioning capabilities without prior assumptions regarding origin of imaging deviations. Further, we demonstrate that the obtained scan pattern allows analysis of these errors, and allows to restore anatomical accuracy relevant for complementary methods such as motion correction, further enhancing spatial registration and feature extraction.

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Language(s): eng - English
 Dates: 2022-062022-07
 Publication Status: Issued
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000827275100007
 Degree: -

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Title: Biomedical Optics Express
  Abbreviation : Biomed Opt Express
Source Genre: Journal
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Publ. Info: Washington, DC : Optical Society of America (OSA)
Pages: - Volume / Issue: 13 (7) Sequence Number: - Start / End Page: 3983 - 3992 Identifier: CoNE: https://pure.mpg.de/cone/journals/resource/2156-7085