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  Photoactivatable Large Stokes Shift Fluorophores for Multicolor Nanoscopy

Likhotkin, I., Lincoln, R., Bossi, M. L., Butkevich, A., & Hell, S. W. (2023). Photoactivatable Large Stokes Shift Fluorophores for Multicolor Nanoscopy. Journal of the American Chemical Society, 145(3), 1530-1534. doi:10.1021/jacs.2c12567.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000C-AB01-2 Version Permalink: https://hdl.handle.net/21.11116/0000-000C-AB02-1
Genre: Journal Article

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jacs.2c12567.pdf (Publisher version), 6MB
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 Creators:
Likhotkin, Ilya, Author
Lincoln, Richard, Author
Bossi, Mariano Luis1, Author           
Butkevich, Alexey1, Author           
Hell, Stefan W.1, Author                 
Affiliations:
1Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, ou_3350048              

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 Abstract: We designed caging-group-free photoactivatable live-cell permeant dyes with red fluorescence emission and ∼100 nm Stokes shifts based on a 1-vinyl-10-silaxanthone imine core structure. The proposed fluorophores undergo byproduct-free one- and two-photon activation, are suitable for multicolor fluorescence microscopy in fixed and living cells, and are compatible with super-resolution techniques such as STED (stimulated emission depletion) and PALM (photoactivated localization microscopy). Use of photoactivatable labels for strain-promoted tetrazine ligation and self-labeling protein tags (HaloTag, SNAP-tag), and duplexing of an imaging channel with another large Stokes shift dye have been demonstrated.

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Language(s): eng - English
 Dates: 2023-01-10
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1021/jacs.2c12567
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Project name : The research was funded by Bundesministerium für Bildung und Forschung (German Federal Ministry of Education and Research), Project No. 13N14122 “3D Nano Life Cell” (to S.W.H.).
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Title: Journal of the American Chemical Society
  Other : JACS
  Abbreviation : J. Am. Chem. Soc.
Source Genre: Journal
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Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: 145 (3) Sequence Number: - Start / End Page: 1530 - 1534 Identifier: ISSN: 0002-7863
CoNE: https://pure.mpg.de/cone/journals/resource/954925376870