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  Modular Assembly of Synthetic Secondary Chromosomes

Zumkeller, C., Schindler, D., & Waldminghaus, T. (2018). Modular Assembly of Synthetic Secondary Chromosomes. In BACTERIAL CHROMATIN: Methods and Protocols (pp. 71-94).

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Item Permalink: https://hdl.handle.net/21.11116/0000-000D-0781-9 Version Permalink: https://hdl.handle.net/21.11116/0000-000D-0782-8
Genre: Conference Paper

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 Creators:
Zumkeller, Celine1, Author
Schindler, Daniel2, Author                 
Waldminghaus, Torsten1, Author
Affiliations:
1external, ou_persistent22              
2LOEWE-Center for Synthetic Microbiology (SYNMIKRO), Philipps-Universität Marburg, Germany, ou_persistent22              

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 Abstract: The development of novel DNA assembly methods in recent years has paved
the way for the construction of synthetic replicons to be used for basic
research and biotechnological applications. Questions of how chromosomes
need to be constructed to maintain the genetic information can now be
answered by a learning-by-building approach. Here, we describe an
efficient pipeline for the design and assembly of synthetic, secondary
chromosomes in Escherichia coli based on the popular Modular Cloning
system (MoClo).

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 Dates: 2018
 Publication Status: Issued
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 Rev. Type: -
 Identifiers: ISI: 000695668800006
DOI: 10.1007/978-1-4939-8675-0_5
 Degree: -

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Title: BACTERIAL CHROMATIN: Methods and Protocols
Source Genre: Proceedings
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Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 71 - 94 Identifier: ISSN: 1064-3745
ISBN: 978-1-4939-8675-0; 978-1-4939-8674-3

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Title: Methods in Molecular Biology
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Pages: - Volume / Issue: 1837 Sequence Number: - Start / End Page: - Identifier: ISSN: 1064-3745