English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
 Local TagsRelease HistoryDetailsSummary
  Molecular basis and design principles of switchable front-rear polarity and directional migration in Myxococcus xanthus

Carreira, L. A. M., Szadkowski, D., Lometto, S., Hochberg, G. K. A., & Søgaard-Andersen, L. (2023). Molecular basis and design principles of switchable front-rear polarity and directional migration in Myxococcus xanthus. Nature Communications, 14(1): 4056. doi:10.1038/s41467-023-39773-y.

Item is

 

show all hide all

Basic

show hide
Item Permalink: https://hdl.handle.net/21.11116/0000-000D-6B26-1 Version Permalink: https://hdl.handle.net/21.11116/0000-000F-2045-F
Genre: Journal Article

Files

show Files

Locators

show
hide
Locator:
https://doi.org/10.1038/s41467-023-39773-y (Publisher version)
Description:
-
OA-Status:
Gold

Creators

show
hide
 Creators:
Carreira, Luis Antonio Menezes1, Author           
Szadkowski, Dobromir1, Author           
Lometto, Stefano2, Author           
Hochberg, Georg K. A.2, Author                 
Søgaard-Andersen, Lotte1, Author                 
Affiliations:
1Bacterial Adaption and Differentiation, Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266305              
2Max Planck Research Group Evolutionary Biochemistry, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266300              

Content

show
hide
Free keywords: -
 Abstract: During cell migration, front-rear polarity is spatiotemporally regulated; however, the underlying design of regulatory interactions varies. In rod-shaped Myxococcus xanthus cells, a spatial toggle switch dynamically regulates front-rear polarity. The polarity module establishes front-rear polarity by guaranteeing front pole-localization of the small GTPase MglA. Conversely, the Frz chemosensory system, by acting on the polarity module, causes polarity inversions. MglA localization depends on the RomR/RomX GEF and MglB/RomY GAP complexes that localize asymmetrically to the poles by unknown mechanisms. Here, we show that RomR and the MglB and MglC roadblock domain proteins generate a positive feedback by forming a RomR/MglC/MglB complex, thereby establishing the rear pole with high GAP activity that is non-permissive to MglA. MglA at the front engages in negative feedback that breaks the RomR/MglC/MglB positive feedback allosterically, thus ensuring low GAP activity at this pole. These findings unravel the design principles of a system for switchable front-rear polarity.

Details

show
hide
Language(s): eng - English
 Dates: 2022-12-092023-06-282023-07-08
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: URI: https://doi.org/10.1038/s41467-023-39773-y
Other: Carreira2023
DOI: 10.1038/s41467-023-39773-y
 Degree: -

Event

show

Legal Case

show

Project information

show hide
Project name : -
Grant ID : -
Funding program : -
Funding organization : Max Planck Society
Project name : -
Grant ID : -
Funding program : Open Access
Funding organization : Projekt DEA

Source 1

show
hide
Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: London : Nature Publishing Group
Pages: 4056 Volume / Issue: 14 (1) Sequence Number: 4056 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723