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  MINSTED tracking of single biomolecules

Scheiderer, L., von der Emde, H., Hesselink, M., Weber, M., & Hell, S. W. (2024). MINSTED tracking of single biomolecules. Nature Methods, 1-9. doi:10.1038/s41592-024-02209-6.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000E-B32E-5 Version Permalink: https://hdl.handle.net/21.11116/0000-000E-B32F-4
Genre: Journal Article

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 Creators:
Scheiderer, Lukas1, Author           
von der Emde, Henrik, Author
Hesselink, Mira, Author
Weber, Michael, Author
Hell, Stefan W.1, Author                 
Affiliations:
1Optical Nanoscopy, Max Planck Institute for Medical Research, Max Planck Society, ou_2364730              

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 Abstract: Here we show that MINSTED localization, a method whereby the position of a fluorophore is identified with precisely controlled beams of a STED microscope, tracks fluorophores and hence labeled biomolecules with nanometer/millisecond spatiotemporal precision. By updating the position for each detected photon, MINSTED recognizes fluorophore steps of 16 nm within <250 μs using about 13 photons. The power of MINSTED tracking is demonstrated by resolving the stepping of the motor protein kinesin-1 walking on microtubules and switching protofilaments.

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Language(s): eng - English
 Dates: 2023-09-152024-02-132024-03-13
 Publication Status: Published online
 Pages: 15
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 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41592-024-02209-6
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Title: Nature Methods
  Abbreviation : Nat Methods
Source Genre: Journal
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Publ. Info: New York, NY : Nature Publishing Group
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 1 - 9 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556