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  Multiple levels of transcriptional regulation control glycolate metabolism in Paracoccus denitrificans

Schada v. Borzyskowsi, L., Hermann, L., Kremer, K., Barthel, S., Pommerenke, B., Glatter, T., et al. (2024). Multiple levels of transcriptional regulation control glycolate metabolism in Paracoccus denitrificans. bioRxiv: the preprint server for biology, doi: 10.1101/2024.03.11.584432.

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2024.03.11.584432v1.full.pdf (Preprint), 6MB
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Schada v. Borzyskowsi, Lennart1, 2, Author           
Hermann, Lucas2, Author
Kremer, Katharina1, Author           
Barthel, Sebastian1, Author           
Pommerenke, Bianca1, Author           
Glatter, Timo3, Author                 
Paczia, Nicole4, Author                 
Bremer, Erhard2, Author
Erb, Tobias J.1, Author                 
Affiliations:
1Understanding and Building Metabolism, Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266303              
2external, ou_persistent22              
3Core Facility Mass Spectrometry and Proteomics, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266266              
4Core Facility Metabolomics and small Molecules Mass Spectrometry, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266267              

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 Abstract: The hydroxyacid glycolate is a highly abundant carbon source in the environment. Glycolate is produced by unicellular photosynthetic organisms and excreted at petagram scales to the environment, where it serves as growth substrate for heterotrophic bacteria. In microbial metabolism, glycolate is first oxidized to glyoxylate by the enzyme glycolate oxidase. The recently described β-hydroxyaspartate cycle (BHAC) subsequently mediates the carbon-neutral assimilation of glyoxylate into central metabolism in ubiquitous Alpha- and Gammaproteobacteria. While the reaction sequence of the BHAC was elucidated in Paracoccus denitrificans, little is known about the regulation of glycolate and glyoxylate assimilation in this relevant alphaproteobacterial model organism. Here, we show that regulation of glycolate metabolism in P. denitrificans is surprisingly complex, involving two regulators, the IclR-type transcription factor BhcR that acts as an activator for the BHAC gene cluster, as well as the GntR-type transcriptional regulator GlcR, a previously unidentified repressor that controls the production of glycolate oxidase. Furthermore, an additional layer of regulation is exerted at the global level, which involves the transcriptional regulator CceR that controls the switch between glycolysis and gluconeogenesis in P. denitrificans. Together, these regulators control glycolate metabolism in P. denitrificans, allowing the organism to assimilate glycolate together with other carbon substrates in a simultaneous fashion, rather than sequentially. Our results show that the metabolic network of Alphaproteobacteria shows a high degree of flexibility to react to the availability of multiple substrates in the environment.Competing Interest StatementThe authors have declared no competing interest.

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Language(s): eng - English
 Dates: 2024-03-11
 Publication Status: Issued
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 Rev. Type: No review
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Title: bioRxiv : the preprint server for biology
  Abbreviation : bioRxiv
Source Genre: Journal
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Pages: - Volume / Issue: - Sequence Number: doi: 10.1101/2024.03.11.584432 Start / End Page: - Identifier: ZDB: 2766415-6
CoNE: https://pure.mpg.de/cone/journals/resource/2766415-6