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  Supramolecular complex of cucurbit[7]uril with diketopyrrolopyrole dye: fluorescence boost, biolabeling and optical microscopy

Kim, D., Bossi, M. L., Belov, V. N., & Hell, S. W. (2024). Supramolecular complex of cucurbit[7]uril with diketopyrrolopyrole dye: fluorescence boost, biolabeling and optical microscopy. Angewandte Chemie International Edition, e202410217, pp. 1-9. doi:10.1002/anie.202410217.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000F-7294-9 Version Permalink: https://hdl.handle.net/21.11116/0000-000F-7298-5
Genre: Journal Article

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https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.202410217 (Any fulltext)
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 Creators:
Kim, Dojin, Author
Bossi, Mariano L.1, Author           
Belov, Vladimir N., Author
Hell, Stefan W.1, Author                 
Affiliations:
1Optical Nanoscopy, Max Planck Institute for Medical Research, Max Planck Society, ou_2364730              

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Free keywords: Cucurbituril • Diketopyrrolopyrrole • Supramolecular Chemistry • Fluorescence • STED microscopy
 Abstract: New photostable and bright supramolecular complexes based on cucurbit[7]uril (CB7) host and diketopyrrolopyrole (DPP) guest dyes having two positively charged 4-(trimethylammonio)phenyl groups were prepared; with spectra (H2O, abs. / emission max. 480 / 550 nm; e ~ 19 000, tfl > 4 ns), strong binding with hosts (~560 nM Kd) and a linker affording fluorescence detection of bioconjugates with antibody and nanobody. Combination of protein-functionalized DPP dye with CB7 improves photostability and affords up to 12-fold emission gain. Two-color confocal and stimulated emission depletion (STED) microscopy with 595 nm or 655 nm STED depletion lasers shows that the presence of CB7 not only leads to improved brightness and image quality, but also results in DPP becoming cell-permeable.

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Language(s): eng - English
 Dates: 2024-06-17
 Publication Status: Published online
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/anie.202410217
 Degree: -

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Title: Angewandte Chemie International Edition
  Abbreviation : Angew. Chem., Int. Ed.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: - Sequence Number: e202410217 Start / End Page: 1 - 9 Identifier: ISSN: 1433-7851
CoNE: https://pure.mpg.de/cone/journals/resource/1433-7851