English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse
  1. View item / 
  2. Item Summary

Item

ITEM ACTIONSEXPORT
Add to Basket
  Local TagsRelease HistoryDetailsSummary

Released
Journal Article

Supramolecular complex of cucurbit[7]uril with diketopyrrolopyrole dye: fluorescence boost, biolabeling and optical microscopy

MPS-Authors
/persons/resource/persons14883

Bossi,  Mariano L.
Optical Nanoscopy, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons15210

Hell,  Stefan W.       
Optical Nanoscopy, Max Planck Institute for Medical Research, Max Planck Society;

External Resource

https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.202410217
(Any fulltext)

https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fanie.202410217&file=anie202410217-s1-DPPCB7_SI_accepted_article.pdf
(Supplementary material)

https://onlinelibrary.wiley.com/doi/epdf/10.1002/anie.202410217
(Any fulltext)

Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Kim, D., Bossi, M. L., Belov, V. N., & Hell, S. W. (2024). Supramolecular complex of cucurbit[7]uril with diketopyrrolopyrole dye: fluorescence boost, biolabeling and optical microscopy. Angewandte Chemie International Edition, e202410217, pp. 1-9. doi:10.1002/anie.202410217.


Cite as: https://hdl.handle.net/21.11116/0000-000F-7294-9
Abstract
New photostable and bright supramolecular complexes based on cucurbit[7]uril (CB7) host and diketopyrrolopyrole (DPP) guest dyes having two positively charged 4-(trimethylammonio)phenyl groups were prepared; with spectra (H2O, abs. / emission max. 480 / 550 nm; e ~ 19 000, tfl > 4 ns), strong binding with hosts (~560 nM Kd) and a linker affording fluorescence detection of bioconjugates with antibody and nanobody. Combination of protein-functionalized DPP dye with CB7 improves photostability and affords up to 12-fold emission gain. Two-color confocal and stimulated emission depletion (STED) microscopy with 595 nm or 655 nm STED depletion lasers shows that the presence of CB7 not only leads to improved brightness and image quality, but also results in DPP becoming cell-permeable.