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  Modular Assembly of Synthetic Secondary Chromosomes

Zumkeller, C., Schindler, D., Felder, J., & Waldminghaus, T. (2024). Modular Assembly of Synthetic Secondary Chromosomes. In R. T. Dame (Ed.), Bacterial Chromatin. Methods in Molecular Biology, (2819, pp. 157-187). Humana, New York, NY.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000F-9768-2 Version Permalink: https://hdl.handle.net/21.11116/0000-000F-977C-C
Genre: Book Chapter

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 Creators:
Zumkeller, Celine1, Author
Schindler, Daniel2, Author                 
Felder, Jennifer1, Author
Waldminghaus, Torsten1, Author
Affiliations:
1external, ou_persistent22              
2Core Facility MPG MAXGenesys DNAfoundry, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266268              

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 Abstract: The development of novel DNA assembly methods in recent years has paved the way for the construction of synthetic replicons to be used for basic research and biotechnological applications. A learning-by-building approach can now answer questions about how chromosomes must be constructed to maintain genetic information. Here we describe an efficient pipeline for the design and assembly of synthetic, secondary chromosomes in Escherichia coli based on the popular modular cloning (MoClo) system.

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Language(s): eng - English
 Dates: 2024-07-20
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: URI: https://doi.org/10.1007/978-1-0716-3930-6_9
Other: Zumkeller2024
DOI: 10.1007/978-1-0716-3930-6_9
 Degree: -

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Title: Bacterial Chromatin. Methods in Molecular Biology,
Source Genre: Book
 Creator(s):
Dame, Remus T., Editor
Affiliations:
-
Publ. Info: Humana, New York, NY, 2819
Pages: - Volume / Issue: 2819 Sequence Number: - Start / End Page: 157 - 187 Identifier: DOI: 10.1007/978-1-0716-3930-6_9