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  Two functionally redundant isoforms of Drosophila melanogaster eukaryotic initiation factor 4B are involved in cap-dependent translation, cell survival and proliferation

Hernandez, G., Vazquez-Pianzola, P., Zurbriggen, M., Altmann, M., Sierra, J. M., & Rivera-Pomar, R. (2004). Two functionally redundant isoforms of Drosophila melanogaster eukaryotic initiation factor 4B are involved in cap-dependent translation, cell survival and proliferation. European Journal of Biochemistry, 271(14): doi:10.1111/j.1432-1033.2004.04217.x, pp. 2923-2926. Retrieved from http://www3.interscience.wiley.com/cgi-bin/fulltext/118751380/HTMLSTART.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-ED0C-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-DA90-2
Genre: Journal Article

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 Creators:
Hernandez, G.1, Author              
Vazquez-Pianzola, P.1, Author              
Zurbriggen, M., Author
Altmann, M., Author
Sierra, J. M., Author
Rivera-Pomar, R.2, Author              
Affiliations:
1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              
2Department of Cellular Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578576              

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Free keywords: cell survival; Drosophila; eukaryotic initiation factor 4B (eIF4B); proliferation; translation
 Abstract: Eukaryotic initiation factor (eIF) 4B is part of the protein complex involved in the recognition and binding of mRNA to the ribosome. Drosophila eIF4B is a single-copy gene that encodes two isoforms, termed eIF4B-L (52.2 kDa) and eIF4B-S (44.2 kDa), generated as a result of the alternative recognition of two polyadeynlation signals during transcription termination and subsequent alternative splicing of the two pre-mRNAs. Both eIF4B mRNAs and proteins are expressed during the entire embryogenesis and life cycle. The proteins are cytoplasmic with polarized distribution. The two isoforms bindRNAwith the same affinity. eIF4B-L and eIF4B-S preferentially enhance cap-dependent over IRES-dependent translation initiation in a Drosophila cellfree translation system. RNA interference experiments suggest that eIF4B is required for cell survival, although only a modest reduction in rate of protein synthesis is observed. Overexpression of eIF4B in Drosophila cells in culture and in developing eye imaginal discs promotes cell proliferation.

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Language(s): eng - English
 Dates: 2004-07-052004-06-23
 Publication Status: Published in print
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Title: European Journal of Biochemistry
Source Genre: Journal
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Pages: - Volume / Issue: 271 (14) Sequence Number: doi:10.1111/j.1432-1033.2004.04217.x Start / End Page: 2923 - 2926 Identifier: -