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High-throughput method for determination of seed paternity by microsatellite markers

MPG-Autoren
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Bhattacharya,  Samik
Department of Molecular Ecology, Prof. I. T. Baldwin, MPI for Chemical Ecology, Max Planck Society;

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Baldwin,  Ian Thomas
Department of Molecular Ecology, Prof. I. T. Baldwin, MPI for Chemical Ecology, Max Planck Society;

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Bhattacharya, S., & Baldwin, I. T. (2013). High-throughput method for determination of seed paternity by microsatellite markers. Bio-protocol, 3(8), 1-12. doi:10.21769/BioProtoc.452.


Zitierlink: https://hdl.handle.net/11858/00-001M-0000-0014-F4C6-0
Zusammenfassung
In this protocol, determination of seed paternity by microsatellite markers in Nicotiana attenuata is described. However, this does not include a protocol for the novel marker selection/identification, but rather exploits the markers generated for a closely related species N. tabacum (Bindler et al., 2007). This is a high-throughput protocol optimized and streamlined for one skilled person to process 384 (96 x 4) seeds in 5 days, from DNA isolation (from seedlings) to paternity assessment by microsatellite genotype data.