Help Privacy Policy Disclaimer
  Advanced SearchBrowse




Journal Article

Stable expression of cloned rat GABAA receptor subunits in a human kidney cell line


Seeburg,  Peter H.
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available

Hamilton, B. J., Lennon, D. J., Im, H. K., Im, W. B., Seeburg, P. H., & Carter, D. B. (1993). Stable expression of cloned rat GABAA receptor subunits in a human kidney cell line. Neuroscience Letters, 153(2), 206-209. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/7687050.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0019-AA7A-E
A predominant form of the GABAA/benzodiazepine receptor-Cl- channel complex is believed to consist of three different 48-55 kDa subunits (alpha, beta, gamma) with unknown stoichiometry. Plasmids containing the rat GABAA receptor cDNAs coding for alpha 1, beta 2, and gamma 2 were co-transfected, along with a plasmid encoding G418 resistance, into human embryonic kidney cells previously transformed with Adenovirus 5 (HEK-293) [J. Gen. Virol., 36 (1977) 59-72]. Four percent of the G418 resistant colonies were found to express mRNA for all three of the GABAA subunits constitutively. A single cell clone derived from one of the alpha 1 beta 2 gamma 2 expressors has demonstrated stable electrophysiological characteristics over 25 passages. The GABA-activated Cl- current in this cell line is blocked by picrotoxin and bicuculline, and is modulated by a variety of agonist and inverse agonist ligands including diazepam, Ro 154513, zolpidem, and beta-CCE. The cell line has been used successfully over a 12-month period as a screen for novel drugs modulating GABA-mediated polarization of neuronal cells.