Fast manipulation of cellular cAMP level by light in vivo

Schröder-Lang, Saskia; Schwärzel, Martin; Seifert, Reinhard; Strünker, Timo; Kateriya, Suneel; Looser, Jens; Watanabe, Masakatsu; Kaupp, Benjamin; Hegemann, Peter; Nagel, Georg

doi:10.1038/nmeth975

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Fast manipulation of cellular cAMP level by light in vivo

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Schröder-Lang, Saskia
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Nagel, Georg
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;
Universität Würzburg, Julius-von-Sachs-Institut für Biowissenschaften, Würzburg, 97082, Germany;

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Schröder-Lang, S., Schwärzel, M., Seifert, R., Strünker, T., Kateriya, S., Looser, J., Watanabe, M., Kaupp, B., Hegemann, P., & Nagel, G. (2007). Fast manipulation of cellular cAMP level by light in vivo. Nature Methods, 4(1), 39-42. doi:10.1038/nmeth975.

引用: https://hdl.handle.net/11858/00-001M-0000-0024-D89F-C

要旨

The flagellate Euglena gracilis contains a photoactivated adenylyl cyclase (PAC), consisting of the flavoproteins PACα and PACβ. Here we report functional expression of PACs in Xenopus laevis oocytes, HEK293 cells and in Drosophila melanogaster, where neuronal expression yields light-induced changes in behavior. The activity of PACs is strongly and reversibly enhanced by blue light, providing a powerful tool for light-induced manipulation of cAMP in animal cells.