A presequence-binding groove in Tom70 supports import of Mdl1 into mitochondria.

Melin, J.; Kilisch, M.; Neumann, P.; Lytovchenko, O.; Gomkale, R.; Schendzielorz, A.; Schmidt, B.; Liepold, T.; Ficner, R.; Jahn, O.; Rehling, P.; Schulz, C.

doi:10.1016/j.bbamcr.2015.04.021

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A presequence-binding groove in Tom70 supports import of Mdl1 into mitochondria.

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http://www.sciencedirect.com/science/article/pii/S0167488915001470/pdfft?md5=8bcedda706420ef9947b1b6591079de7&pid=1-s2.0-S0167488915001470-main.pdf
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Melin, J., Kilisch, M., Neumann, P., Lytovchenko, O., Gomkale, R., Schendzielorz, A., et al. (2015). A presequence-binding groove in Tom70 supports import of Mdl1 into mitochondria. Biochimica et Biophysica Acta-Molecular Cell Research, 1853(8), 1850-1859. doi:10.1016/j.bbamcr.2015.04.021.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0027-BA93-D

Abstract

The translocase of the outer mitochondrial membrane (TOM complex) is the general entry gate into mitochondria for almost all imported proteins. A variety of specific receptors allow the TOM complex to recognize targeting signals of various precursor proteins that are transported along different import pathways. Aside from the well-characterized presequence receptors Tom20 and Tom22 a third TOM receptor, Tom70, binds proteins of the carrier family containing multiple transmembrane segments. Here we demonstrate that Tom70 directly binds to presequence peptides using a dedicated groove. A single point mutation in the cavity of this pocket (M551R) reduces the presequence binding affinity of Tom70 ten-fold and selectively impairs import of the presequence-containing precursor Mdl1 but not the ADP/ATP carrier (MC). Hence Tom70 contributes to the presequence import pathway by recognition of the targeting signal of the Mdl1 precursor.