English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse
  1. Basket / 
  2. Item Summary

Item

ITEM ACTIONSEXPORT
Add to Basket
  Local TagsRelease HistoryDetailsSummary
 PreviousNext  

Released
Journal Article

Reversibility and viscoelastic properties of micropillar supported and oriented magnesium bundled F-actin

MPS-Authors
/persons/resource/persons75812

Maier,  Timo
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

/persons/resource/persons75557

Haraszti,  Tamas
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

External Resource

http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0136432&type=printable
(Any fulltext)

http://dx.doi.org/10.1371/journal.pone.0136432
(Any fulltext)

Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Maier, T., & Haraszti, T. (2015). Reversibility and viscoelastic properties of micropillar supported and oriented magnesium bundled F-actin. PLoS One, 10(8): e0136432, pp. 1-17. doi:10.1371/journal.pone.0136432.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0028-50B6-8
Abstract
Filamentous actin is one of the most important cytoskeletal elements. Not only is it responsible for the elastic properties of many cell types, but it also plays a vital role in cellular adhesion and motility. Understanding the bundling kinetics of actin filaments is important in the formation of various cytoskeletal structures, such as filopodia and stress fibers. Utilizing a unique pillar-structured microfluidic device, we investigated the time dependence of bundling kinetics of pillar supported free-standing actin filaments. Microparticles attached to the filaments allowed the measurement of thermal motion, and we found that bundling takes place at lower concentrations than previously found in 3-dimensional actin gels, i.e. actin filaments formed bundles in the presence of 5-12 mM of magnesium chloride in a time-dependent manner. The filaments also displayed long term stability for up to hours after removing the magnesium ions from the buffer, which suggests that there is an extensive hysteresis between cation induced crosslinking and decrosslinking.