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Journal Article

Simultaneous dual-color fluorescence lifetime imaging with novel red-shifted fluorescent proteins

MPS-Authors

Laviv,  Tal
Max Planck Florida Institute for Neuroscience, Max Planck Society;

Yasuda,  Ryohei
Max Planck Florida Institute for Neuroscience, Max Planck Society;

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Citation

Laviv, T., Kim, B. B., Chu, J., Lam, A. J., Lin, M. Z., & Yasuda, R. (2016). Simultaneous dual-color fluorescence lifetime imaging with novel red-shifted fluorescent proteins. Nature Methods, advance online publication, 989-992. doi:10.1038/nmeth.4046.


Cite as: https://hdl.handle.net/11858/00-001M-0000-002E-2AE0-A
Abstract
We describe a red-shifted fluorescence resonance energy transfer (FRET) pair optimized for dual-color fluorescence lifetime imaging (FLIM). This pair utilizes a newly developed FRET donor, monomeric cyan-excitable red fluorescent protein (mCyRFP1), which has a large Stokes shift and a monoexponential fluorescence lifetime decay. When used together with EGFP-based biosensors, the new pair enables simultaneous imaging of the activities of two signaling molecules in single dendritic spines undergoing structural plasticity.