Direct expression in Escherichia coli of a DNA sequence coding for human growth 
hormone

Goeddel, David V.; Heyneker, Herbert L.; Horzumi, T.; Arentzen, Rene; Itakura, Keiichi; Yansura, Daniel G.; Ross, Michael J.; Miozzari, Guiseppe; Crea, Roberto; Seeburg, Peter H.

doi:10.1038/281544a0

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone

MPS-Authors

/persons/resource/persons95292

Seeburg, Peter H.
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

External Resource

https://www.nature.com/articles/281544a0.pdf
(Any fulltext)

https://doi.org/110.1038/281544a0
(Any fulltext)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Goeddel, D. V., Heyneker, H. L., Horzumi, T., Arentzen, R., Itakura, K., Yansura, D. G., et al. (1979). Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone. Nature, 281(5732), 544-548. doi:10.1038/281544a0.

Cite as: https://hdl.handle.net/21.11116/0000-0001-22E5-6

Abstract

DNA coding for human growth hormone was constructed by using chemically synthesised DNA in conjunction with enzymatically prepared cDNA. This 'hybrid' gene was expressed in Escherichia coli under the control of the lac promoter. A polypeptide was produced having the size and immunological properties characteristic of mature human growth hormone.