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Collision Cross Sections and Ion Mobility Separation of Fragment Ions from Complex N-Glycans


Pagel,  Kevin
Molecular Physics, Fritz Haber Institute, Max Planck Society;
Institut für Chemie und Biochemie, Freien Universität BerlinTakustrasse 3, 14195, Berlin, Germany;

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Harvey, D. J., Watanabe, Y., Allen, J. D., Rudd, P., Pagel, K., Crispin, M., et al. (2018). Collision Cross Sections and Ion Mobility Separation of Fragment Ions from Complex N-Glycans. Journal of the American Society for Mass Spectrometry. doi:10.1007/s13361-018-1930-1.

Ion mobility mass spectrometry (IM- MS) holds great potential for structural glycobiology, in particular in its ability to resolve glycan isomers. Generally, IM-MS has largely been applied to intact glycoconjugate ions with reports focusing on the separation of different adduct types. Here, we explore IM separation and report the collision cross section (CCS) of complex type N-glycans and their fragments in negative ion mode following collision-induced dis- sociation (CID). CCSs of isomeric fragment ions were found, in some cases, to reveal structural details that were not present in CID spectra themselves. Many fragment ions were confirmed as possessing multiple structure, details of which could be obtained by comparing their drift time profiles to different glycans. By using fragmen- tation both before and after mobility separation, information was gathered on the fragmentation pathways producing some of the ions. These results help demonstrate the utility of IM and will contribute to the growing use of IM-MS for glycomics.